1310 nm傅里叶域眼前节OCT的成像特点及与其他两种OCT的对比分析

目的 探讨1310 nm傅里叶域实时眼前节光学相干断层扫描(OCT)的成像特点及与RTV-ue100 OCT,Visante OCT对眼前节重要结构的成像能力的对比.方法 横断面研究.选择受试者6例(12眼,24个方位),运用1310 nm傅里叶域实时眼前节OCT,RTV-ue100 OCT与Visante OCT进行房角成像,对巩膜突、Schlemm's管、房角隐窝及Schwalbe线进行定义,测量前房角角度(TIA500)、500 μm前房角开放距离(AOD500)、500 μm小梁虹膜面积(TISA500)、Schlemn's管长径以及面积.运用Fisher's确切概率法对比3台仪器对房角重要结构的定义能力,并采用独立样本t检验研究三者生物学测量结果的差异.结果 傅里叶域实时眼前节OCT可在8眼(67％)13方位(54％)、12眼(100％)22方位(92％)、12 眼(100％)24方位(100％)及6眼(50％)9方位(38％)分别定义巩膜突、Schlemm's管、房角隐窝及Schwalbe线.RTV-ue100 OCT可于8眼(67％)8方位(33％)定义Schlemm's管,10眼(83％)19方位(79％)定义Schwalbe线,不能定义巩膜突与房角隐窝.Visante OCT于12眼(100％)21方位(88％)定义巩膜突,12眼(100％)24方位(100％)定义房角隐窝,不能定义Schlemm's管与Schwalbe线.3种OCT对巩膜突、Schlemm's管、房角隐窝、Schwalbe线定义能力差异有统计学意义(F=24.30、25.20、28.22、17.10,P＜0.01).傅里叶域实时眼前节OCT与Visante OCT测量TIA500、AOD500、TISA500的差异无统计学意义.傅里叶域实时眼前节OCT与RTV-ue100 OCT测量Schlemm's管面积、长径差异无统计学意义.结论 三种OCT都实现了前房角的清晰成像,显示角膜、巩膜、结膜以及巩膜突、Schlemm's管与Schwalbe线等房角结构.RTV-ue OCT和傅里叶域实时眼前节OCT的图像分辨率较高,能够显示Schlemm’s管与Schwalbe线等细微结构;Visante OCT和傅里叶域实时眼前节OCT深层结构检测能力强,各设备所得生物学参数无明显差异.

The imaging characteristics and comparative analysis of 1310 nm study of real-time Fourierdomain anterior segment OCT

Objective To discuss the imaging ability of different anterior optical coherence tomography （OCT） systems. Methods It was a cross-sectional study. Six normal volunteers （12 eyes, 24 directions） were involved in this cross-sectional study. Three different OCT systems were used to acquire images of their eyes. The important structures： scleral spur, Schlemm＇s canal, anterior segment angle recess and Schwalbe line were defined. The trabecular-iris angle （TIA500）, angle opening distance at 500 um （AOD500）, the area of the trabeculo-iris space at 500 um （TISA500）, the widest diameter of Schlemm＇s canal, and the area and circumference of Schlemm＇s canal were measured if possible. Results Using real-time Fourier-domain anterior segment OCT, the scleral spur, Schlemm＇s canal, anterior segment angle recess and the Schwalbe line were defined in 8 eyes （67%） 13 positions （54%）, 12 eyes （100%） 22 positions （92%）, 12 eyes （100%） 24 positions （100%）, and 6 eyes （50%） 9 positions （38%）, respectively. Using RTV-ue 100 OCT, Schlemm＇s canal and the Schwalbe line could be defined in 8 eyes （67%） 8 positions （33%） and 10 eyes （83%） 19 positions （79%）, respectively.The scleral spur and anterior segment angle recess could not be seen. The scleral spur and anterior segment angle recess were defined in 12 eyes （100%） 21 positions （88%） and 12 eyes （100%） 24 positions （100%）, respectively using Visante OCT, but Schwalbe＇s line and Schlemm＇s canal could not be defined. There was a significant difference in their ability to define important structures （scleral spur, P〈0.01; Schlemm＇s canal, P〈0.01; anterior segment angle recess, P〈0.01; and Schwalbe line, P〈0.01）. The TIA500, AOD500 and TISA500 measured by Real-time Fourier-domain anterior segment OCT and Visante OCT were similar, and the area and widest diameter of Schlemm＇s canal measured by Real-time Fourier-domain anterior segment OCT and RTV-ue 100 OCT were also similar. The differences were not significant. Conclusion All three OCTs can acquire clear images of the anterior segment angle. Real-time Fouriel-domain anterior segment OCT and RTV-ue 100 OCT can acquire high-resolution images and are good at imaging subtle structures. Real-time Fourier-domain anterior segment OCT and Visante OCT are good at imaging deep structures.