一种改良的蛋白质电泳考马斯亮蓝G-250染色方法

目的建立一种简便、低毒的蛋白质聚丙烯酰胺凝胶电泳染色方法。方法电泳后的凝胶经短暂固定后,用含氨基磺酸的考马斯亮蓝G-250溶液染色,无需脱色。结果该方法灵敏度高于经典考马斯亮蓝R-250染色方法,并去除了常规染色和脱色液中有毒的甲醇等溶剂。结论改良方法具有灵敏度高、染色背景浅、节省染料、直接显色无需脱色及配方安全低毒等优点。

An improved method for protein staining with CBBG-250 following electrophoresis

Objective To establish a simple and less toxic method for protein staining following SDS-PAGE.Methods After electrophoresis,the gel was fixed temporarily,and then stained with the Coomassie Brilliant Blue G-250(CBBG-250) solution containing aminosulfonic acid.De-staining was not required.Results The sensitivity of this method was higher than that of the classical CBBR-250 staining method.Moreover,toxic solvents such as methanol,though commonly used in the staining and de-staining solutions,were eliminated.Conclusions The improved method has a series of advantages including high sensitivity,clear background,dye-saving,direct color development without de-staining,and having a safe and less toxic recipe.