人脐带间充质干细胞抑制同种异体脐血淋巴细胞的转化

目的探讨人脐带间充质干细胞(UCMSCs)的生物学特性及其对同种异体脐血淋巴细胞转化的影响。方法从人脐带中分离、培养得到间充质干细胞,通过免疫细胞化学染色等方法对其进行表型鉴定;同时采用MTT法检测UCMSCs对同种异体脐血淋巴细胞转化的影响,以验证UCMSCs在免疫调控方面的作用。结果成功培养出UCMSCs,培养至第3代的UBMSCs形态为均一梭形,细胞倍增时间为51.6 h。细胞表面表达MSCs相关抗原CD29,但不表达造血细胞相关抗原CD34,细胞化学染色显示PAS(+)、NBE(+)、POX(-),表明此种自脐带中分离得到并诱导扩增的成纤维样细胞为间充质干细胞;UCMSCs对PHA刺激的脐血淋巴细胞,增殖反应具有免疫抑制作用,且该作用与UCMSCs接种数量有关,当接种量为2 000个/孔时,抑制率高达60.8%。结论 UCMSCs具有免疫抑制作用,能抑制同种异体脐血淋巴细胞的转化,且此种抑制作用与接种的脐血淋巴细胞数量有关。

Umbilical cord mesenchymal stem cells inhibit the transformation of lymphocytes from allogeneic umbilical cord blood

Objective To study the biological characteristics of mesenchymal stem cells(MSCs) derived from human cord(UCMSCs) and the inhibitory effect on the transformation of lymphocytes from allogeneic Umbilical Cord Blood.Methods UCMSCs were isolated from umbilical cord.The effect of UCMSCs on the transformation of lymphocytes from allogeneic UCB was tested by MTT.ResultsThe morphological feature of the third passage’s UCMSCs(P3-UCMSCs) displayed an uniform fibroblast-like phenotype.The doubling time of P3-MSCs was 51.6 hours and expressed CD29 but not CD34.The histochemical staining of P3-UCMSCs showed that PAS and NBE were positive but POX was negative.It indicated that the fibroblast-like cells induced and expanded from human cord in our experiment were MSCs;UCB-lymphocyte transformation was suppressed when UCMSCs were cultured together with UCB-lymphocytes and the suppression effect was related with UCMSCs numbers plated in eath well,the suppression rate reached 60.8% when 2 000 UCMSCs were plated.Conclusions UCMSCs had an immunomodulatory function.UCMSCs may suppress the transformation of lymphocytes from allogeneic UCB and the suppression function seemed dependent on MSC numbers plated in each well.