| 肿瘤细胞p53突变状况对p53基因治疗的影响 |
| |
| 引用本文： | 赵刚,苏伟,肖刚,周新平,孙建华,黄美雄. 肿瘤细胞p53突变状况对p53基因治疗的影响[J]. 肿瘤防治研究, 2010, 37(3): 301-304. DOI: 10.3971/j.issn.1000-8578.2010.03.016 |
| |
| 作者姓名： | 赵刚 苏伟 肖刚 周新平 孙建华 黄美雄 |
| |
| 作者单位： | 100730卫生部北京医院胃肠外科 |
| |
| 摘 要： | 目的探讨肿瘤细胞p53突变状况对p53基因治疗的影响。方法PCR-SSCP及DNA测序分析MCA-205和Panc-02细胞的p53基因突变状况；流式细胞计数了解腺病毒载体对这两种细胞的转导情况；TUNEL方法分析转导p53基因后的细胞凋亡情况，培养细胞计数分析对离体肿瘤细胞生长的影响；利用小鼠肝转移肿瘤模型，分析Adv-p53对于在体肿瘤的抗肿瘤作用。结果MCA-205细胞p53基因的第769个碱基由G突变为T，Panc-02细胞未发现p53基因突变。腺病毒载体在Panc-02细胞有较高的转导率，而Adv-p53在MCA-205细胞诱导更明显的细胞凋亡。Adv-p53在离体细胞培养试验中抑制MCA-205细胞的生长，而没有明显抑制Panc-02细胞的生长。在小鼠肝转移肿瘤模型中，门静脉注射Adv-p53明显抑制MCA205肝转移肿瘤的生长，而在Panc-02肝转移肿瘤中抗肿瘤作用不明显。结论Adv-p53的抗肿瘤作用在有p53基因突变的肿瘤细胞中效果明显，肿瘤细胞p53突变状况对p53基因治疗有一定影响。
|
| 关 键 词： | Adv-p53 p53基因突变 抗肿瘤作用 |
| 收稿时间： | 2008-10-09 |
| 修稿时间： | 2009-01-08 |
Influence of p53 Mutation Status in Tumor Cells to Gene Therapy of Adv-p53 |
| |
| ZHAO Gang,SU Wei,XIAO Gang,ZHOU Xin-ping,SUN Jian-hua,HUANG Mei-xiong. Influence of p53 Mutation Status in Tumor Cells to Gene Therapy of Adv-p53[J]. Cancer Research on Prevention and Treatment, 2010, 37(3): 301-304. DOI: 10.3971/j.issn.1000-8578.2010.03.016 |
| |
| Authors: | ZHAO Gang SU Wei XIAO Gang ZHOU Xin-ping SUN Jian-hua HUANG Mei-xiong |
| |
| Affiliation: | Department of General Surgery, Beijing Hospital, Beijing 100730, China |
| |
| Abstract: | Objective To investigate the influence of p53 mutation status in tumor cells to the gene therapy of Adv-p53.Methods The mutation status of p53 gene in MCA-205 and Panc-02 cells was analyzed by SSCP-PCR and DNA sequence.The transduction ratio of adenovirus vectors was analyzed by flow cytometric analysis.Apoptosis of tumor cells transfected with Adv-p53 was analyzed by TUNEL assay.Anti-tumor effect of Adv-p53 was examined in murine liver metastasis model.Results p53 gene in MCA-205 cells had a mutation in 76... |
| |
| Keywords: | Adv-p53 Adv-p53 p53 mutation status Anti-tumor effect |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《肿瘤防治研究》浏览原始摘要信息 |
|
点击此处可从《肿瘤防治研究》下载免费的PDF全文 |
