| 呼吸道合胞病毒不同感染时间对过敏性气道炎症反应的作用研究 |
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| 引用本文： | 张雷英,王冰,那立新,闫建忠,刘北星.呼吸道合胞病毒不同感染时间对过敏性气道炎症反应的作用研究[J].中国免疫学杂志,2011,27(2):177-180. |
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| 作者姓名： | 张雷英 王冰 那立新 闫建忠 刘北星 |
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| 作者单位： | 1. 中国医科大学基础医学院免疫学教研室,沈阳,110001
2. 沈阳市皇姑区疾病控制中心,沈阳,110001 |
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| 基金项目： | 辽宁省教育厅科研项目基金(2009A745)资助 |
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| 摘 要： | 目的:探讨呼吸道合胞病毒(RSV)不同感染模式对花粉诱导的气道过敏性炎症反应的影响。方法:用花粉抽提物JCP致敏BALB/c鼠,在致敏前或致敏后的不同时间点经鼻粘膜感染RSV。HE染色法分类肺泡灌洗液中炎症细胞类型并计数其数量;ELISA法检测血清中过敏原特异性抗体含量;细胞因子检测试剂盒检测肺组织细胞培养上清中IL-17和IL-5水平。结果:与对照JCP组相比,JCP致敏前RSV感染组肺组织炎症细胞浸润程度明显减轻,炎症细胞总数及嗜酸性粒细胞数量明显下降;血清IgE总量及JCP特异性IgE和IgG1抗体水平显著降低;肺组织淋巴细胞IL-17分泌水平升高,而IL-5分泌减少。与致敏前RSV感染模式不同,致敏后RSV感染无论是对JCP诱导的气道过敏性炎症反应,亦或对血清抗体水平和细胞因子分泌活性均无明显影响。结论:RSV不同感染模式对JCP诱导的过敏性气道炎症具有不同的作用特点,致敏前RSV感染降低过敏原诱导的气道炎症反应。
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| 关 键 词： | RSV感染 花粉抽提物(JCP) 炎症 IL-17 IL-5 |
Timing of respiratory syncytial virus infection on regulation of allergic airway inflammation |
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| ZHANG Lei-Ying,WANG Bing,NA Li-Xin,YAN Jian-Zhong,LIU Bei-Xing.Timing of respiratory syncytial virus infection on regulation of allergic airway inflammation[J].Chinese Journal of Immunology,2011,27(2):177-180. |
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| Authors: | ZHANG Lei-Ying WANG Bing NA Li-Xin YAN Jian-Zhong LIU Bei-Xing |
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| Institution: | ZHANG Lei-Ying,WANG Bing,NA Li-Xin,YAN Jian-Zhong,LIU Bei-Xing.Department of Immunology,School of Basic Medical Science China Medical University,Shenyang 110001,China |
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| Abstract: | Objective:To investigate the timing features for respiratory syncytial virus(RSV) infection on regulation of JCP-induced allergic airway inflammation.Methods:BALB/c mice were intranasally infected with RSV before or after sensitization to JCP.Number of inflammatory cells in the BALs was counted by HE staining.JCP-specific antibody and total IgE titer in the sera were measured using a mouse antibody quantitative kit.Cytokine production of IL-17 and IL-5 in the supernatant of cultured lung parenchyma cells we... |
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| Keywords: | Respiratory syncytial virus infection Japanese cedar pollen crude extract Inflammation IL-17 IL-5 |
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