| 急性冠脉综合征患者胸腺新近输出CD4~+CD25~+调节性T细胞的检测及意义 |
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| 引用本文： | 马旭明,苏又苏,方红成,谢培益,何少林,林静,赵卉,黎明,昌薇,李大主.急性冠脉综合征患者胸腺新近输出CD4~+CD25~+调节性T细胞的检测及意义[J].中国免疫学杂志,2011,27(2):172-176. |
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| 作者姓名： | 马旭明 苏又苏 方红成 谢培益 何少林 林静 赵卉 黎明 昌薇 李大主 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院心内科,武汉,430022
2. 广东医学院附属深圳南山医院心内科,深圳,518052 |
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| 基金项目： | 国家自然基金资助项目(No.30670855) |
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| 摘 要： | 目的:探讨急性冠脉综合征患者胸腺新近输出的CD4+CD25+调节性T细胞(Treg)的水平及意义。方法:实验共分3组:急性冠脉综合征(ACS)组28例,稳定型心绞痛(SA)组25例和健康对照(HCs)组24例。采用CD31作为胸腺新近输出的典型标志,用磁性细胞分离器(MACS)分离各组CD4+CD25+调节性T细胞,流式细胞分析法检测各组患者外周血CD31+Treg占Treg细胞的比例,应用实时定量PCR检测CD4+CD25+调节性T细胞中T细胞受体重排删除环的数量。结果:ACS组外周血CD31+Treg/Treg比例明显低于SA组(P=0.003)和HCs组(P<0.001)。ACS组Treg细胞中TREC的数量亦明显低于SA组(P=0.001)和HCs组(P<0.001)。而SA组和HCs组间差异均无统计学意义(P=0.106和P=0.566)。患者CD31+Treg的比例与Treg细胞中TREC的数量呈正相关(r=0.493,P=0.014)。结论:急性冠状动脉综合征患者胸腺新近输出CD4+CD25+调节性T细胞减少可能是动脉粥样斑块不稳定的原因。
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| 关 键 词： | 急性冠脉综合征 CD4+CD25+调节性T细胞 胸腺新近输出 CD31 TREC |
Significance of the change in CD4+CD25+ regulatory T cells of recent thymic emigrants in acute coronary syndromes |
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| MA Xu-Ming,SU You-Su,FANG Hong-Cheng,XIE Pei-Yi,HE Shao-Lin,LIN Jing,ZHAO Hui,LI Ming,CHANG Wei,LI Da-Zhu.Significance of the change in CD4+CD25+ regulatory T cells of recent thymic emigrants in acute coronary syndromes[J].Chinese Journal of Immunology,2011,27(2):172-176. |
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| Authors: | MA Xu-Ming SU You-Su FANG Hong-Cheng XIE Pei-Yi HE Shao-Lin LIN Jing ZHAO Hui LI Ming CHANG Wei LI Da-Zhu |
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| Institution: | MA Xu-Ming,SU You-Su,FANG Hong-Cheng,XIE Pei-Yi,HE Shao-Lin,LIN Jing,ZHAO Hui,LI Ming,CHANG Wei,LI Da-Zhu.Department of Cardiology,Institute of Cardiovascular Diseases,Union Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430022,China |
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| Abstract: | Objective:To investigate the alteration and its significance of recent thymic emigrant CD4+CD25+regulatory T cells in patient with acute coronary syndrome.Methods:77 subjects were divided into three groups:acute coronary syndromes(ACS,n=28),stable angina pectoris(SA,n=25) and healthy controls(HCs,n=24).The frequencies of recent thymic emigrant CD4+CD25+regulatory T cells(Treg) in peripheral blood were detected by flow cytometry.The number of T-cell receptor excision circles(TREC) in Treg was determined by r... |
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| Keywords: | Acute coronary syndromes CD4 CD25 Treg Recent thymic emigrants CD31 TREC |
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