日本血吸虫循环抗原的生化检测

Ｓｊ６５蛋白为来源于日本血吸虫虫卵的循环抗原，该蛋白免疫原性较弱，能与一种分子量为６８ＫＤ的糖蛋白（Ｓｊ６５结合蛋白）专一结合。利用酶标记Ｓｊ６５结合蛋白，建立Ｄｏｔ－ＥＬＩＳＡ检测感染血清中的Ｓｊ６５。用该法测定急、慢性血吸虫病人血清，阳性率分别为１００％及８１．３％。正常人、兔、鼠、肝炎病人血清及蛔、钩虫病人血清均未见阳性反应。在感染兔血清及并殖吸虫病人血清中可检出Ｓｊ６５，而在感染小鼠血清及并殖吸虫感染犬血清中却未检出。感染兔经吡喹酮治疗５周后，９０％转为阴性。慢性血吸虫病人经吡喹酮治疗１年后，６０％转为阴性。结果表明，感染血清中Ｓｊ６５的生化检测似能反映感染程度及考核治疗效果，同时也提示，血清中Ｓｊ６５的水平可因宿主的不同而不同。

BIOCHEMICAL DEETECTION OF THE CIRCULATING ANTIGEN OF SCHISTOSOMA JAPONICUM

The circulating antigen,Sj65 protein ,was prepared from eggs of Schistosomajaponicum. It has weak immunogenicity and has a binding protein with M.W. 68kD. Using thebinding protein labeled with HRP,we established a method of detecting Sj 65 in infected serum.The sera from acute and chronic stages of schistosomiasis patients were screened by this method,the positive rates were 100%and 81.3%, respectively. The sera from normal persons and personswith hepatitis, ascariasis and ancylostomiasis all gave negative reaction. The Sj65 could be detected in sera from rabbits infected with S. japonicum and from persons infected with paragonimus,but the Sj65 could not be detected in sera from mice infected with S. japonicum and from dogs in-fected with paragonimus. The 90%of infected rabbits become negative reactions five weeks afterpraziquantel treatment,60%of patients become negative one year after praziquantel treatment,These results showed that the level of Sj65 was correlated with intensity of infection and efficacyof treatment,it also suggested that levels of Sj65 in different hosts were different.