碱性成纤维细胞生长因子对豚鼠耳蜗螺旋神经节细胞谷氨酸钠毒性损伤的保护作用

目的　在体研究碱性成纤维细胞生长因子 (bFGF)对谷氨酸钠所致豚鼠耳蜗螺旋神经节细胞毒性损伤的拮抗作用。方法　谷氨酸钠组 :谷氨酸钠 2g·kg-1·d-1,ip× 18d ;bFGF组 :bFGF 80 0U·kg-1·d-1,ip ,谷氨酸钠 2g·kg-1·d-1,ip× 18d。停止用药后耳廓反射粗略测定动物听觉水平 ,后取耳蜗 ,固定包埋、切片染色 ,光镜观察。结果　谷氨酸钠组表现为基底转与顶转几乎均匀一致的耳蜗螺旋神经节细胞的消失 ,平均细胞密度为 (1976 3± 10 18 9)个 /mm2 ,占正常的 4 5 % ;bFGF组细胞密度为 (384 0 9± 373 1)个 /mm2 ,约为正常组的 89%。经方差分析 ,F 检验 ,谷氨酸钠组与bFGF组比较 ,耳蜗螺旋神经节存活细胞密度差异显著 ,P <0 0 5。结论　bFGF对豚鼠谷氨酸钠耳蜗旋神经节细胞在体毒性损伤具有保护作用

The protective effect of bFGF on guinea pig cochlear spiral ganglion cell against glutamate neurotoxicity

Objective To study the antitoxic effect of basic fibroblast growth factor(bFGF)on survival and neurogenesis of the spiral ganglion cell of guinea pig exposed to glutamate in vivo.Methods Glutamate group was injected monosodium glutamate 2 g·kg -1 ·d -1 i.p. for 18d;bFGF group was injected bFGF 800 U·kg -1 ·d -1 and monosodium glutamate 2 g·kg -1 ·d -1 i.p.for 18d.After that,PR of both groups of guinea pig were tested,and the cochlear spiral ganglion cells were also detected by microscoe.Results In glutamate group,the spiral ganglion cells almost disappeared,frome base to apex.The density of cochlear spiral ganglion cells were (1976 3±1018 9)/mm 2(45%)in bFGF group the density of spiral ganglion cell were (3840 9±373 1)/mm 2(89%),P<0 05.Conclusion bFGF has protective effect on guinea pig cochlea spiral ganglion cell injury induced by glutamate.