毛囊干细胞表皮膜片修复裸鼠皮肤缺损的实验研究

目的开发新的组织工程表皮替代物,利用毛囊干细胞-壳聚糖明胶膜片(Chitosan-gelatin membrane,CGM)进行修复裸鼠皮肤缺损的实验研究。方法将体外扩增培养的毛囊干细胞接种于CGM,构建组织工程表皮膜片,裸鼠背部作直径1cm的全层皮肤缺损,将毛囊干细胞-CGM复合物覆盖创面。回植后1周、4周和12周分别进行组织学和免疫组织化学检测。结果毛囊干细胞在CGM上生长良好。毛囊干细胞-CGM修复裸鼠后1周切片示创面有新生上皮覆盖,表皮分化良好,而对照组残留较大未愈创面。修复后4周和12周可见对照组收缩较实验组显著。结论体外构建的毛囊干细胞-CGM可以修复裸鼠皮肤缺损。

An Experimental study of human hair follicle stem cells- chitosan-gelatin membrane for repairing the skin defect of nude mouse

Objective To develop a new tissue engineering（TE）-epidermal substitute by using the hair follicle stem cells-chitosan-gelatin membrane（CGM） to repair skin defects of the nude mouse. Methods A full-thickness skin defect was made on the dorsum of nude mouse. The hair follicle stem cells were inoculated and on the CGM, the hair follicle stem cells-CGM was constructed to repair the skin defects of nude mouse. A full-thickness skin defect was only covered with chitosan-gelatin membrane in the control group. The nude mice were sacrificed 1,4,12 weeks respectively after the operation. The grafts were examined by HE and immunohistology staining. Results The hair follicle stem cells grow well on the surface of CGM. 1 week after operation, a new epidermal covered the surface of the skin defects. HE showed the well- differentiated keratinocytes of new epidermal; The unhealed wound was found 4,12 weeks after transplantation in the control group and the contraction of the skin was significantly greater than the experimental group. Conclusion The hair follicle stem cells-chitosan-gelatin membrane is an ideal cell source for repairing the skin defect in nude mouse.