AQP1、AQP2及AQP3在人腺性膀胱炎组织中的表达

目的：研究水通道蛋白（aquaporin,AQP）1、2、3在人腺性膀胱炎组织中的分布及表达情况。方法：取经尿道腺性膀胱炎电切手术切取的腺性膀胱炎组织及周围正常膀胱组织各30例,应用免疫组织化学S-P法检测AQP1、AQP2及AQP3的分布及表达，并通过计算机对染色结果进行灰度值测定。结果：在腺性膀胱炎组织中AQP1主要表达在微血管内皮细胞，免疫组化阳性指数35.947；AQP2主要表达在炎性膀胱黏膜上皮细胞的胞膜及胞质内，免疫组化阳性指数34.644；AQP3则呈阴性表达。AQP1在正常膀胱组织中主要表达于膀胱黏膜下微血管和小动脉的内皮细胞, AQP2和AQP3表达于膀胱黏膜上皮细胞的细胞膜及胞质中。AQP1、AQP2及AQP3在正常膀胱组织的免疫组化阳性指数分别为57.038、61.425及60.468，与在腺性膀胱炎组织中的表达比较差异有显著性（P<0.01）。结论：腺性膀胱炎时AQPs的表达量明显减低，炎症病变影响AQPs在膀胱组织中的的表达。

Expressions of AQP1, AQP2 and AQP3 in human cystitis glandularis tissue

Objective To study the distribution and expressions of aquaporin(AQP)1,2,3 in human cystitis glandularis tissue.Methods The expressions and distribution of AQP1,AQP2,AQP3 in 30 cases of cystitis glandularis tissue and 30 cases of normal bladder tissue were detected by immunohistochemistry technique,and the gray scale values of the dyeing consequence were examined by computer.Results In cystitis glandularis tissue AQP1 mainly expressed in the endothelial cells of micrangium,the immunohistochemical positive exponent was 35.947;AQP2 expressed in the cell membrane and periplasm of the mucosa epithelial cells,the immunohistochemical positive exponent was 34.644;and AQP3 did not express.In normal bladder tissue,AQP1 mainly expressed in the endothelial cells of capillary and arteriolar under the mucous membrane of urinary bladder,AQP2 and AQP3 expressed in the celluar membrane and periplasm of the mucous membrane of urinary bladder.The immunohistochemical positive exponent of AQP1,AQP2,AQP3 in the normal bladder tissue were 57.038,61.425 and 60.468,respectively,there were significant differences compared with AQPs in the cystitis glandularis tissue(P<0.01).Conclusion The expressions of AQPs are obviously reduced in cystitis glandularis tissue,and the inflammation affects the expression of AQPs.