| 大肠癌组织P21~(WAF1/CIP1)的表达与p53基因突变的关系 |
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| 引用本文： | 殷智榕,张云汉,姜国忠,高冬玲,宋一民.大肠癌组织P21~(WAF1/CIP1)的表达与p53基因突变的关系[J].河南大学学报(医学版),2001,20(2):1-3. |
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| 作者姓名： | 殷智榕 张云汉 姜国忠 高冬玲 宋一民 |
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| 作者单位： | 河南医科大学一附院,病理科,河南,郑州,450052 |
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| 摘 要： | 目的 :探讨大肠癌组织P2 1WAF1/CIP1的表达与p5 3基因突变的关系及其意义。方法 :应用RT -PCR、RCR -SSCP方法对 42例新鲜大肠癌组织中上述两种基因的表达进行研究。结果 :p5 3基因在大肠癌组织中的突变率为47.6 % (2 0 / 42 ) ,且在中、低分化大肠癌组织中的突变北显著高于高分化者 (P <0 .0 5 ) ;P2 1WAF1/CIP1在中、低分化大肠癌组织中的表达显著低于高分化癌组织 (P <0 .0 1)。 2 0例p5 3基因突变组中 ,P2 1WAF1/CIP1表达阳性率明显低于 2 2例p5 3基因未突变组 (P <0 .0 5 )。结论 :大肠癌组织中P2 1WAF1/CIP1的低表达可能与p5 3基因突变有关。
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| 关 键 词： | 大肠癌 P21WAF1/CIP1 p53 RT-PCR RCR-SSCP |
| 文章编号： | 1008-2867(2001)02-0001-03 |
| 修稿时间： | 2000年12月21 |
Relationship between the expression of P21WAF1/CIP1 and p53 genomic mutation in colorectal carcinoma |
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| YIN Zhi_rong,ZHANG Yun_han,JIANG Guo_zhong,et al.Relationship between the expression of P21WAF1/CIP1 and p53 genomic mutation in colorectal carcinoma[J].Journal of Henan University,2001,20(2):1-3. |
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| Authors: | YIN Zhi_rong ZHANG Yun_han JIANG Guo_zhong |
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| Abstract: | Objective To investigate the relationship between the expression of P21 WAF1/CIP1 and p53 genomic mutation in colorectal carcinoma. Methods RT_PCR, PCR_SSCP was used to study two_gene expressions above of 42 cases of colorectal carcinoma fresh tissue.Results The mutation rate of p53 in carcinoma tissue was 47.6%(20/42), and mutation rate in poorly or moderately differentiated colorectal carcinoma was significantly higher than that of highly differentiated P <0.05, but the expression of P21 WAF1/CIP1 in the former was lower than the latter P <0.01, and the expression of P21 WAF1/CIP1 in group of 20 case of p53 mutation was lower than that in group of 22 case of p53 non-mutation( P <0.05) Conclusion Low expression of P21 WAF1/CIP1 in colorectal carcinoma was probably related to the mutation of p53 gene. |
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| Keywords: | colorectal carcinoma P21 WAF1/CIP1 p53 RT-PCR PCR_SSCP |
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