胰岛素对血管内皮细胞衰老及Akt表达的研究

目的:探讨胰岛素对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)衰老及Akt表达的影响。方法:不同浓度的胰岛素(对照组、1 nmol/L、10 nmol/L、胰岛素100 nmol/L)加入体外培养的人脐静脉内皮细胞,24 h后β半乳糖酶染色检测细胞衰老率和免疫组化检测Akt活性。结果:与对照组比较,1 nmol/L组和10 nmol/L组细胞衰老程度降低,Akt表达上调。但随着浓度增加,100 nmol/L组细胞衰老反而加重。而Akt表达无统计学差异。结论:低浓度胰岛素可延缓内皮细胞衰老,可能与Akt基因表达上调有关。而高浓度胰岛素加重细胞衰老,其机制可能与Akt蛋白无关。

Study on effects of insulin on senescence and Akt expression of endothelial cell

Objective：To investigate the effects of insulin on senescence of human umbilical vein endothelial eell （HUVEC） and Akt expression. Methods：The HUVEC were incubated with different concentrations of insulin （control group, 1 nmol/L group, 10 nmol/L group,100nmol/L group）. 24 hours later,the cell senescence rate was measured by SA-β-gal staining, and the expression of Akt was measured by immunohistochemistry. Results： Compared to the control group, the cellular senescence rate of 1 nmol/L and 10 nmol/L group decreased,while Akt expression increased. But with the increasing of insulin concentrations, the cellular senescence rate of 100 nmol/L insulin group increased obviously,and Akt expression unchanged. Conclusion：Low concentration insulin might inhibit human umbilical vein endothelial cells senescence ,which is related with the increase of Akt expression. High dose of insulin might enhance cel-lular senescence,which might be unrelated to Akt protein.