冠心病患者外周血晚期内皮祖细胞集落数量与功能的变化

目的探讨冠心病患者外周血晚期内皮祖细胞集落数量和功能的变化。方法入选研究对象54例,分成冠心病组(n=27)和对照组(n=27)。密度梯度离心法从外周血中获得单个核细胞,体外培养扩增21天后鉴定内皮祖细胞,计数晚期集落数量,分别用MTT比色法、改良的Boyden小室法和HFN培养板测定晚期内皮祖细胞的增殖、迁移和黏附功能。结果冠心病组内皮祖细胞晚期集落数量(2.5±1.2)与对照组(3.8±1.6)相比,明显减少(P<0.05);增殖、迁移和黏附能力与对照组比较(分别为0.324±0.024比0.433±0.064,9.9±2.5比13.9±4.1,21.3±5.1比31.0±7.1)显著降低(P<0.05)。结论冠心病患者晚期内皮祖细胞的数量减少,功能受损。

Changes in the Amount and Function of the Late Endothelial Progenitor Cells Colonies Derived from Peripheral Blood in Patients with Coronary Heart Disease

Aim To explore changes in the amount and function of the late endothelial progenitor cells(EPC) colonies derived from peripheral blood in patients with coronary heart disease(CHD).Methods Fifty four cases were divided into CHD group(n=27) and control group(n=27).Mononuclear cells were isolated from peripheral venous blood with density-gradient centrifugation and were cultured.After 21 days,the late EPC were identified and the late EPC colonies were counted.The late EPC proliferation and migration were assay...