热休克蛋白70在第三磨牙成牙本质细胞中表达的增龄变化

目的观察热休克蛋白70（heat shock protein70 HSPT0）在第三磨牙成牙本质细胞中表达的增龄变化。方法共选取33例21—30岁、31～40岁、4I～50岁和51～60岁四个年龄组健康第三磨牙的组织标本进行免疫组化染色，并通过计算机图像分析对比四组牙髓组织成牙本质细胞中HSP70表达量的不同。结果41～50岁和51～60岁年龄组平均光密度值明显低于21～30岁年龄组和31～40岁年龄组（P〈0．05），其余各组间无显著性差异。结论以41～50岁牟龄组为转折点，HSP70在第三磨牙成牙本质细胞中的表达强度显著下降。

Age-related alterations in expression of HSP70 in third molar odontoblasts

Objective To evaluate age-related changes in the expression of HSP70 in odontoblasts of third molars.Methods Thirty-three healthy third molars divided into 4 groups; 21～30 years old, 31～40 years old, 41～50 years old and 51～60 years old, were collected. Immunohistochemistry staining was performed with mouse anti-HSP70 monoclonal antibody. The expression intensity of HSP70 in odontoblasts was assessed by image analysis system.Results The average optical density (AOD) values of 41～50 years old group and 51～60 years old group were lower than 21～30 years old group and 31～40 years old group significantly（P＜0.05）. There was no statistical differences between the other two groups.Conclusion After 41～50 years old, the expression intensity of HSP70 in odontoblasts of third molars declines significantly.