| 胃癌端粒酶活性及端粒酶hTRT表达的研究 |
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| 引用本文： | 徐萍,厉有名,王开明,王升启,郑晓飞.胃癌端粒酶活性及端粒酶hTRT表达的研究[J].浙江大学学报(医学版),2000,29(2):70-72,81. |
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| 作者姓名： | 徐萍 厉有名 王开明 王升启 郑晓飞 |
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| 作者单位： | 1. 浙江大学医学院附属第一医院，浙江 杭州310003
2. 军事医学科学院放射医学研究所，北京100850 |
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| 摘 要： | 目的：探讨端粒酶活性及端粒酶ｈＴＲＴ表达在胃癌发和琢发展中的作用。方法“分别采用ＴＦＡＰ银染法和ＲＴ－ＰＣＲ法对胃粘膜粒粒酶活性及端粒酶ｈＴＲＴ ｍＲＮＡ进行检测。结果：胃癌-%一酶阳性率为１００％（８／８），胃癌前状态组织是性率为４０％４／１０），浅表性胃炎均为阴性（０／９）。胃癌组织ｈＴＲＴ ｍＲＮＡ阳性率为８５．７％（６／７），浅表性胃炎均为阴性（０／５）。结论：端粒酶激活参与胃癌的形成和发展
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| 关 键 词： | 端粒酶 hTRT基因 基因表达 胃肿瘤 |
| 文章编号： | 1008-9292(2000)02-0070-03 |
Telomerase Activity and hTRT Gene Expression in Gastric Cancer |
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| XU Ping,LI You min,WANG Kai ming,et al.Telomerase Activity and hTRT Gene Expression in Gastric Cancer[J].Journal of Zhejiang University(Medical Sciences),2000,29(2):70-72,81. |
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| Authors: | XU Ping LI You min WANG Kai ming |
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| Abstract: | Objective:To investigate the role of telomerase activity and hTRT gene expression in the process of gastric carcinogenesis.Methods:Telomerase activity in gastric mucosa specimens was exami ned by PCR based telomeric repeats amplification protocol (TRAP) assay,and hTRT gene expression in gastric mucosa was measured by semi quantitative RT PCR assays of hTRT mRNA.Results:Telomerase activity was detected in all of the gastric neoplasm specimens (8/8),and 4 of 10 precancerous state specimens,while no telomerase activity in 9 chronic superficial gastritis.Six of 7 specimens with gastric neoplasms revealed high levels of the expression of hTRT ,while the expression was not detected in 5 chronic superficial gastritis.Conclusion:Telomerase activation may be a critical step in gastric carcinogenesis.The detection of telomerase may be helpful in the early diagnosis of gastric cancers.The expression levels of hTRT may correlate with telomerase activity in gastric mucosa. |
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| Keywords: | Telomerase hTRT gene Gene expression Stomach neoplasms/enzymol Silver staining Polymerase chain reaction |
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