| HIV-2gp105重组疫苗联合免疫引起小鼠更强的免疫应答 |
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| 引用本文： | 李子健,金宁一,张立树,江文正,张洪勇,李昌.HIV-2gp105重组疫苗联合免疫引起小鼠更强的免疫应答[J].吉林大学学报(医学版),2006,32(1):1-3. |
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| 作者姓名： | 李子健 金宁一 张立树 江文正 张洪勇 李昌 |
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| 作者单位： | 军事医学科学院军事兽医研究所,全军基因工程重点实验室,吉林,长春,130062;北京大学第三医院血管医学研究所,北京,100083;军事医学科学院军事兽医研究所,全军基因工程重点实验室,吉林,长春,130062;华东师范大学生命科学学院,上海,200433 |
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| 基金项目： | 国家科技攻关项目
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吉林省科技厅科技发展计划 |
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| 摘 要： | 目的：探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。方法：将HIV-2 gp105重组DNA疫苗与重组鸡痘病毒以prime-boost免疫策略，免疫BALB/c小鼠，检测免疫小鼠脾CD4+、CD8+ T细胞亚群的数量、脾特异性CTL杀伤活性和血清抗
体滴度。结果：重组DNA疫苗和重组鸡痘病毒prime-boost免疫策略和两种疫苗单独免疫均刺激小鼠产生针对HIV-2靶细胞的特异性CTL杀伤活性及特异性抗体，联合免疫组特异性CTL杀伤活性及特异性抗体水平高于单独免疫组(P<0.05)。结论：以HIV-2 gp105重组DNA疫苗进行基础免疫、以HIV-2 gp105重组鸡痘病毒进行加强免疫的prime-boost免疫策略能诱导小鼠产生更强的特异性细胞和体液免疫应答。
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| 关 键 词： | HIV-2 gp105 疫苗 DNA 重组鸡痘病毒 免疫策略 |
| 文章编号： | 1671-587X(2006)01-0001-03 |
| 收稿时间： | 2005-03-16 |
| 修稿时间： | 2005年3月16日 |
Higher immune response inflicted by combined immunization of HIV-2 gp105 |
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| LI Zi-jian,JIN Ning-yi,ZHANG Li-shu,JIANG Wen-zheng,ZHANG Hong-yong,LI Chang.Higher immune response inflicted by combined immunization of HIV-2 gp105[J].Journal of Jilin University: Med Ed,2006,32(1):1-3. |
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| Authors: | LI Zi-jian JIN Ning-yi ZHANG Li-shu JIANG Wen-zheng ZHANG Hong-yong LI Chang |
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| Institution: | 1. Key Laboratory of Genetic Engineering of PLA, Military Veterinary Institute, Academy of Military MedicalSciences, Changchun 130062,China；2. Institute of Vascular Medicine,Third Hospital,Beijing University, Beijing 100083,China; 3.School of Life Science,East China Normal University,Shanghai 200433,China |
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| Abstract: | Objective To provide fundamental data for developing an effective immunization strategy by different types of recombinant vaccines to protect HIV-2 infection. Methods Using prime-boost immunization strategy, BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus. The numbers of CD4 and CD8 subgroup of spleen T lymphocyte, the specific killing activities of spleen CTL and the liters of serum antibodies of the immunized mice were detected. Results HIV-2 specific target-killing activity of spleen CTL and serum HIV-2 specific antibody level were higher in combined immunization group than those in single immunization groups (P<0. 05). Conclusion The strategy of prime-boost may stimulate stronger specific celluar and humoral immune responses in mice. |
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| Keywords: | HIV-2 gp105 vaccines DNA recobinant fowlpox virus immunization strategy prime-boost |
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