Nuclear DNA analysis of oral hyperplasia and dysplasia using image cytometry |
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| Authors: | M. Abdel-Salam B. H. Mayall L. S. Hansen K. L. Chew J. S. Greenspan |
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| Affiliation: | Divisions of Oral Biology, University of California, San Francisco, U.S.A.;Divisions of Oral Pathology, Department of Stomatology, University of California, San Francisco, U.S.A.;Divisions of Lawrence Livermore National Laboratory, Livermore, California, and Department of Laboratory Medicine, University of California, San Francisco, U.S.A. |
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| Abstract: | We investigated the value of image analysis in discriminating among oral white lesions with hyperplasia without dysplasia and oral white or white-and-red lesions with moderate or severe dysplasia. Normal oral epithelial tissue was used as a control. Image analysis was applied to 5-μm formalin-fixed sections stained with the azure A-Feulgen reaction for nuclear DNA. For 150–200 cells from each section, 5 nuclear variables were assessed: area, form factor, total stain, average stain and ellipticity. For each variable, 2 measurements were obtained, the mean and the interquartile range, and were used for stepwise discriminant analysis. Using this test, a model of 3 measurements with the most discriminating power was developed. When the jackknife classification test was applied to this model, we could discriminate with 81% accuracy between the 4 groups of tissue studied. |
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