乳腺癌端粒酶活性与PCNA表达关系

目的探讨端粒酶活性与增殖细胞核抗原（PCNA）表达之间的关系及联合检测对乳腺癌术后治疗方案制定和预测预后的意义。方法采用端粒重复序列扩增聚合酶链反应-酶联免疫吸附测定法（TRAP-PCR-ELISA）和免疫组织化学链霉素生物蛋白过氧化物酶（S-P）法对80例乳腺癌组织、36例乳腺癌相应癌旁组织和10例乳腺良性病变的端粒酶活性及增殖细胞核抗原进行检测。结果（1）80例乳腺癌患者癌组织标本、36例乳腺癌相应癌旁组织标本中端粒酶表达阳性率分别为65．0％和8．3％。差异有统计学意义（P〈0．01）；10例乳腺良性病变组织标本中未检测出端粒酶活性；腋窝淋巴结有无转移与端粒酶阳性表达率差异无统计学意义（P〉0．05）。（2）80例乳腺癌组织中PCNA的阳性表达率为63．75％；乳腺癌有淋巴结转移组PCNA阳性表达率显著高于无淋巴结转移组（P〈0．01），且呈正相关（P〈0．01），即淋巴结转移程度越低。PCNA阳性表达率亦越低。（3）端粒酶活性与PCNA表达具有一致性（P〈0．01）。且呈正相关（P〈0．01），一致率为86．25％。结论端粒酶活性与PCNA表达具有一致性，端粒酶活性与PCNA联合检测，可为乳腺癌术后治疗方案的制定和预测预后提供辅助依据。

Relationship between telomerase activation and PCNA expression in breast cancer

Objective To approach the interrelation between telomerase activation and PCNA expression in breast cancer and evaluate the significance of combined detection in formulating therapeutic regimen after breast cancer operation.Methods The level of telomerase activation and PCNA expression were detected in 80 cases of breast cancer,36 cases of side-cancer tissues and 10 cases of lacteal gland benign lesion using TRAP-PCR-ELISA and S-P immunohistochemistry technique.Results(1)The positive rate of telomerase in 80 breast cancer tissues and 36 side-cancer tissues were 65.0% and 8.3% respectively and the difference revealed statistical significance(P<0.01),the telomerase activation was not examinated in 10 cases of lacteal gland benign lesion;It is no significance in statistic that the level of positive rate of telomerase and the extent of axillary nodes metastasis(P>0.05).(2)Among 80 cases,the positive rate of PCNA in breast cancer tissues was 63.75% and the positive rate of PCNA in group with lymphatic metastasis was significantly higher than that in group without lymphatic metastasis(P<0.01) and there was positive relationship between lymphatic metastasis and positive expression of PCNA(P<0.01).(3)The telomerase activation was in accordance with PCNA expression(P<0.01) and there was positive relationship between telomerase activation and PCNA(P<0.01),the concordance rate was 86.25%.Conclusion The telomerase activation is in accordance with PCNA expression,associating telomerase activation with PCNA detection can provide the ancillary evidence to predict the prognosis and to formulate the treatment plan after breast cancer operation.