| 柴胡疏肝散对肝郁证模型大鼠脑海马磷酸二酯酶-4及其亚型表达的影响 |
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| 引用本文： | 杜雅薇,岳广欣,王蓬文,任映,孙海芸.柴胡疏肝散对肝郁证模型大鼠脑海马磷酸二酯酶-4及其亚型表达的影响[J].中华中医药学刊,2014(1):178-180. |
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| 作者姓名： | 杜雅薇 岳广欣 王蓬文 任映 孙海芸 |
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| 作者单位： | [1]北京中医药大学东直门医院,北京101700 [2]中国中医科学院,北京100053 |
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| 摘 要： | 目的:研究柴胡疏肝散对肝郁证模型大鼠脑海马磷酸二酯酶-4及其亚型表达的影响。方法:将大鼠分为空白对照组、肝郁模型组、柴胡疏肝散组,运用模具束缚结合孤养的方法制备肝气郁结证候动物模型,采用qRT-PCR法检测大鼠脑海马PDE4A、PDE4B及PDE4D表达的影响。结果:模型组大鼠脑海马PDE4A、PDE4B及PDE4D的mRNA表达较正常组降低(P<0.05),柴胡疏肝散组大鼠脑海马PDE4A、PDE4B及PDE4D的mRNA表达较模型组升高(P<0.05)。结论:柴胡疏肝散抗肝郁作用的机制可能与通过上调肝郁大鼠脑海马PDE4及其亚型的mRNA表达而起到保护受损神经元,改善大脑功能,缓解肝郁症状的作用。
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| 关 键 词： | 肝气郁结证 柴胡疏肝散 磷酸二酯酶-4 海马 大鼠 |
Expressions of CHSG Powder on PDE4 and Subtypes in Hippocampus of Rats with Liver- qi Stagnation |
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| DU Yawei,YUE Guangxin,WANG Pengwen,REN Ying,SUN Haiyun.Expressions of CHSG Powder on PDE4 and Subtypes in Hippocampus of Rats with Liver- qi Stagnation[J].Chinese Archives of Traditional Chinese Medicine,2014(1):178-180. |
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| Authors: | DU Yawei YUE Guangxin WANG Pengwen REN Ying SUN Haiyun |
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| Institution: | 1 Dongzhimen Hospital Affiliated to Beijing University of TCM,Beijing 100700,China; 2. China Academy of Chinese Medical Sciences, Beijing 100053, China) |
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| Abstract: | Objective：To observe the expressions of CHSG powder on PDE4 and subtypes in hippocampus of rats with liver - qi stagnation. Methods：The rats were divided into three groups：control, model and CHSG powder groups. This ani- mal model was made by the method of confining the rats＇ movement. The expressions of PDFA and subtypes in hippocam- pus in different groups were observed by qRT - PCR method. Results ： Compared to the control group, the expressions of PDE4A,PDE4B and PDE4D in hippocampus of the model group were decreased （P 〈 0.05 ）. Compared to the model group ,the expressions of PDE4A, PDE4B and PDE4D in hippocampus of the CHSG powder group were increased（ P 〈 0.05 ）. Conclusion： CHSG powder has the function of inhibiting liver - qi stagnation by improving the expressions of PDE4B and subtypes in hippocampus. |
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| Keywords: | liver - qi stagnation CHSG powder phosphodiesterase - 4 （ PDE4 ） hippocampus rat |
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