新生鼠败血症肺脏核因子-κB表达 |
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引用本文: | 潘凤,史源,李华强,覃世文,唐仕芳,赵锦宁. 新生鼠败血症肺脏核因子-κB表达[J]. 中国当代儿科杂志, 2003, 5(5) |
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作者姓名: | 潘凤 史源 李华强 覃世文 唐仕芳 赵锦宁 |
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作者单位: | 第三军医大学大坪医院野战外科研究所儿科,重庆,400042 |
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摘 要: | 目的探讨NF-κB信号途径在新生鼠败血症中的作用,为临床寻求以NF-κB为靶点的治疗手段提供实验依据。方法应用10 d新生大鼠制备金黄色葡萄球菌败血症模型。采用电泳迁移率改变分析(EMSA)方法检测败血症新生鼠肺脏NF-κB的表达情况和应用吡咯-硫氨基甲酸酯(PDTC)后肺脏NF-κB活性变化情况。结果 金黄色葡萄球菌败血症新生鼠的肺脏NF-κB活性于注菌后1 h开始增强,3 h达高峰。PDTC对肺脏NF-κB活化有抑制作用,且剂量越大,抑制作用越强。结论新生鼠金黄色葡萄球菌败血症时肺脏NF-κB有明显激活,且存在一高峰期。抗氧化剂PUTC能对金黄色葡萄球菌败血症新生鼠肺脏NF-κB活化有所抑制,且存在量-效依赖关系。
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关 键 词: | 核因子-κB 败血症 肺 吡咯二硫氨基甲酸酯 新生鼠 |
Activity of Nuclear Factor-κB in the Lungs of Newborn Rats with Sepsis |
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Abstract: | Objective To study the role of NF-κB signal pathway in neonatal rats with sepsis so as to provide the experimental base for corresponding clinical treatment of sepsis, in which NF-icB is taken as the target. Methods The sepsis model was established by injecting staphylococcus aureus subcutaneously in 10-day-old newborn rats. The activity of NF-icB in the lungs of newborn rats with staphylococcus aureus sepsis was detected by the electrophoretic mobility shift assay (EMSA) and the effect of anti-oxidant pyrrolidine dithiocarbamate (PDTC) on it was studied. Results In newborn rats with staphylococcus aureus sepsis, the activity of lung NF-κB was enhanced at the 1st h and reached a peak at the 3rd h after injection of staphylococcus aureus. PDTC had an inhibitive effect on the activity of lung NF-κB. The larger the dosage, the more intensified the inhibitive effect. Conclusions In newborn rats with staphylococcus aureus sepsis, the NF-κB of lungs is activated, and the activation of NF-κB has a peak. The anti-oxidant PDTC can inhibit lung NF-κB activity in a dose-effect way in newborn rats with staphylococcus aureus sepsis. |
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Keywords: | NF-κB Sepsis Lung Pyrrolidine dithiocarbamate Newborn rat |
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