大鼠静脉血栓栓塞症血管内皮生长因子和碱性成纤维细胞生长因子的研究

目的建立大鼠静脉血栓栓塞症（VTE）模型，观察大鼠VTE后血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）的变化。方法sD大鼠78只，随机分为DVT（18只）、DVT-PTE（54只）、正常对照（6只）组。DVT组依据血栓形成时间不同（1，4，7d）分为D1、D4、D7三个亚组；DVT-PTE组依据DVT和PTE时间不同（1，4，7d）分为D1P1、D1P4、D1P7、D4P1、D4P4、D4P7、D7P1、D7P4、D7P7九个亚组，每亚组6只。采用显微血管夹阻滞一侧股静脉血流，制备DVT模型；DVT后不同时间分离有血栓的股静脉，摘取血栓，从另一侧股静脉注入栓子，制备DVT-PTE模型。行肺组织VEGF和bFGF免疫组织化学检查，并测定血浆VEGF和bFGF浓度。结果1、VEGF抗原：对照组主要在支气管上皮细胞胞浆表达，DVT-PTE组第1天肺动脉平滑肌细胞和肺泡上皮细胞胞浆可见表达，第4天肺动脉内皮细胞胞浆可见表达，第4和7天机化栓子中成纤维细胞胞浆有表达。bFGF抗原：对照组主要由支气管平滑肌和肺动脉平滑肌细胞胞浆表达，DVT-VIE组第1天肺动脉平滑肌细胞胞浆表达增强，第4和7天肺动脉周围基质和机化栓子内成纤维细胞胞浆可见表达。2、血清bFGF浓度在D1亚组升高，而在D7、D7P4和D7P7亚组降低，与对照组比较差异均有显著性（P〈0.05）。结论VEGF和bFGF参与VTE血栓机化和血管内膜修复。

Study of the level of vascular endothelial growth factor and basic fibroblast growth factor in a rat model of venous thromboembolism

Objective To investigate the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on the lung and measure their serum level in a rat model of venous thromboembolism (VTE).Methods 78 SD rats were randomly divided into deep vein thrombosis (DVT) (n=18), deep vein thrombosis-pulmonary thromboembolism (DVT-PTE) (n=54) and control group (n=6). Furthermore, the DVT group and DVT-PTE group were divided into 3 and 9 subgroups respectively, according to different duration...