小儿乙型肝炎超微结构与临床

研究小儿乙型病毒性肝炎超微结构和临床特点。方法应用光镜、电镜、免疫组化多克隆HBV（PAP）法，血乙肝标志物用ELISA法，HBV-IgM斑点杂交法结合临床对27例住院患儿肝穿刺活组织标本进行对比观察，并结合临床进行分析。结果在各型乙肝过程中，肝细胞核与细胞器病变均较广泛，且病理诊断常重于临床诊断。结论光镜结合电镜是明确肝炎型别和寻找病因的一种重要方法，能使诊断更趋完善和准确。

Ultrastructure and clinical manifestation of hepatitis B in children

Objective To reaseach the ultrastructure and clinical featuresin children with hepatitis B.Methods Using light microscope, electron microscope,immunohistochemical method of multiclone (PAP), ELISA to markHBV in sero and HBV-IgM spotting hibridization, we studied andcompared 27 liver tissue specimens of children in patient by needlebiopsy,thus we made the clinical analysis.Results In every different stages of hepatitis B,we all find theevidence that the lesion of the nucleas and cell organs in liver wascomparatively extensive, and the pathological diagonosis was oftenmore important than that of the clinical.Conclusions Using light and electron microscope is animportant method to know the type of hepatitis and to find the cause of thedisease,thus to make the diagonosis more perfect and correct.