胃癌HER2蛋白表达及基因扩增分析

目的 探讨胃癌组织HER2蛋白表达和基因扩增情况及其临床意义.方法 收集2011年4月至2012年5月间青岛大学医学院附属医院黄岛院区收治的80例胃癌标本,采用免疫组织化学染色（IHC）和显色原位杂交（CISH） 方法检测HER2蛋白表达和基因扩增情况,分析其与临床病理因素的相关性.结果 80例患者中,HER2蛋白表达（-）51例,（＋）12例,（＋＋）12例,（＋＋＋）5例,阳性表达率为21.3％（17/80）；HER2基因扩增 4例,临界扩增3例,基因扩增率为8.8％（7/80）.IHC和CISH检测结果呈正相关（P＜0.05）,两者的符合率为85.0％（68 /80）.HER2表达与肿瘤部位、分化程度及TNM分期有关（均P＜0.05）.结论 胃癌组织中有较高的HER2蛋白表达和基因扩增率,其与胃癌的发生发展密切相关.

Analysis of the protein expression and gene amplification of HER2 in gastric carcer

Objective To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer.Methods Immunohistochemistry （IHC） and chromogenic in situ hybridization（CISH） method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients.Results Protein expression of HER2 was negative in 51 cases,（＋） in 12 cases,（＋＋） in 12 cases,（＋＋＋） in 5 cases,and the positive expression rate was 21.3%（17/80）.Seven （8.8%） cases had gene amplification of HER2,including gene critical amplification in 3（3.8%） cases.The result of IHC was positively correlated with CISH （P〈0.05）,and the coincidence rate was 85.0% （68/80）.HER2 positive expression rate was higher in the gastroesophageal junction carcinoma,poorly differentiated and stage Ⅲ-Ⅳ gastric cancer （all P〈0.05）.Conclusion The gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2,which is closely correlated to the development of gastric cancer.