RNA干扰畸胎瘤源性生长因子基因对食管鳞癌细胞生物学行为的影响

目的 探讨RNA干扰畸胎瘤源性生长因子（PCDGF）基因对食管鳞癌细胞Eca-109的影响.方法 脂质体介导PCDGF-shRNA的表达载体转染Eca-109细胞,应用RT-PCR、Westernblot检测转染后细胞PCDGF mRNA及蛋白的表达情况,分别采用Counting Kit-8（CCK-8）试剂盒和Boyden小室法检测转染后Eca-109细胞的增殖能力和侵袭能力.结果 转染组PCDGF mRNA和蛋白表达水平均较其他组下调.转染24、48和72 h转染组细胞增殖均受到抑制,抑制率分别为20.4％、21.1％和20.9％,其细胞增殖活性较未转染组、阴性质粒组和脂质体组均明显降低（均 P＜0.05）.未转染组、阴性质粒组、脂质体组和转染组的细胞迁移数分别为118.8±12.0、100.8±9.0、114.3±4.7和 53.5±16.3,转染组与其余3组比较,差异均有统计学意义（P=0.001,P=0.002,P=0.002）.结论 RNA干扰PCDGF基因可抑制食管鳞癌细胞的体外增殖及侵袭能力,PCDGF基因可能成为基因治疗的新靶点.

Effect of PC cell-derived growth factor RNA interference on biological behavior of esophageal squamous carcinoma cells

Objective To investigate the effect of PC cell-derived growth factor （PCDGF） RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.Methods The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome.After transfection,the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively.Cell Counting Kit-8 （CCK-8） assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.Results The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector （transfection group）.Twenty-four,48 and 72 h after transfection,the cells proliferation in the transfection group was inhibited,and the inhibition rate was 20.4%,21.1% and 20.9% respectively.The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group,liposome group and negative vector group （all P〈0.05）.The number of cell migration in the nontransfection group,negative vector group,liposome group and transfection group was 118.8 ±12.0,100.8±9.0,114.3 ±4.7,and 53.5±16.3 respectively.The differences were statistically significant between the transfection group and the other 3 groups （all P〈0.05）.Conclusions PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro.PCDGF gene may be the new target of gene therapy.