家兔循经组织不同温度改变对Na~+/K~+-ATP酶活性及P物质含量的影响

目的 :观察循经温度现象与Na+/K+ ATP酶活性变化及血管调控物质SP功能活动的关系。方法 :①用红外热像技术观察循经出现的温度变化。② )用酶学方法测定Na+/K+ ATP酶活性 ,放射免疫方法测定P物质 (SP )含量。结果 :①左后肢外侧和右后肢内侧循经高温区组织Na+/K+ ATP酶活性均高于对照区组织 (P <0 .0 5、P <0 .0 1 ) ;②左后肢外侧和右后肢内侧循经高温区组织的SP含量较对照区组织有升高趋势 ,但均无统计学差异 (P >0 .5,>0 .1 )。结论 :①家兔循经出现温度较高组织的Na+/K+ ATP酶活性较非高温区组织的代谢活跃 ;②家兔循经高温区组织P物质水平有一定增高 ,但未见统计学意义

Relationship between the Change of Temperature and the Activity of Na+ /K+ -ATPase and Content of SP in the Tissues along the Running Course of Meridians in Rabbits

Objective: To observe the interrelation between moxibustion induced changes of temperature and Na +/K +-ATPase activity as well as SP content in the tissues along the ruuning course of meridians. Methods: Seven healthy mongrel rabbits were used in this study. Before and after moxibustion (5 min) of unilateral "Zusanli" (ST 36) or "Yinlingquan"(SP 9), the cutaneous temperature along the Stomach Meridian was detected with an infra-red thermography. The skin and muscle tissues showing higher temperature along the Stomach Meridian and the Spleen Meridian of the hindlimb part and those of non-higher temperature region (beside the higher-temperature region) were taken respectively under the monitoring of the infra-red thermography. The tissue samples of higher temperature region and non-higher temperature region were taken tobe processed into tissue homogenate, followed by detecting the activity of Na +/K +-ATPase with enzymologic method and the contents of SP(substance P) with radioimmunoassay. Results: ① After moxibustion, the cutaneous temperature increased significantly. μmol Pi/mg prot/hr. The activity values of Na +/K +-ATPase in the higher and non-higher temperature areas were 1.922±0.066 μmol Pi/mg prot/hr and 1.649±0.122 μmol Pi/mg prot/hr at the lateral side of the left hindlimb, and 2.189±0.241 μmol Pi/mg prot/hr and 1.832± 0.132 μmol Pi/mg prot/hr at the medial side of the right hindlimb separately . It shows that the activity of Na +/K +-ATPase in the higher-temperature region is considerably higher than that of the lower temperature area (P<0.05, 0.01); ② The contents of SP in the higher and non-higher temperature areas were 118.92±28.17 pg/mL and 111.39±29.46 pg/mL at the lateral side of the left hindlimb, and 181.06±24.50 pg/mL and 135.74±26.19 pg/mL at the medial side of the right hindlimb separately. It shows that SP contents in the higher-temperature region are higher slightly than those of non-higher temperature region despite no statistical differences between them(P>0.5,0.1). Conclusion: Results suggest that the metabolism of tissues in the higher-temperature region is active and SP may play a minor role in moxibustion-induced higher-temperature phenomenon along the meridian.