兔动脉壁损伤后脂蛋白脂酶的高表达与动脉粥样硬化早期病变形成的关系

目的 :探讨动脉壁脂蛋白脂酶 (LPL)的高表达在动脉粥样硬化发生中的作用。方法 :新西兰兔 16只 ,随机分为 2组。经球囊导管损伤颈总动脉后 ,以腺病毒为载体转移人LPL称实验组及人碱性磷酸酶 (AP)基因为对照组。 7天后通过油红O染色及免疫组化对血管壁的形态进行分析。结果 :应用腺病毒载体有效介导了外源性LPL和AP基因分别在家兔球囊损伤的血管内膜下的高表达。实验组血管壁内膜下有明显的脂质沉积 ,定量分析显示比对照组增高近 3 7倍。同时发现载脂蛋白B在表达LPL的动脉壁内膜下沉积也增加 ,并伴有局部巨噬细胞浸润增多。而两组实验动物的血浆总胆固醇、甘油三酯及高密度脂蛋白胆固醇水平均没有显著差异。结论 :LPL在兔动脉内膜损伤的血管壁局部高表达能够促进动脉粥样硬化早期病变的形成。

Overexpression of Lipoprotein Lipase Promotes Atherosclerosis in a Rabbit Model of Arterial Injury

Objective: To assess the role of lipoprotein lipase( LPL) overexpression in atherogenesis. Methods: The carotid arteries of New Zealand white rabbits were infected with adenovirus containing either human LPL or alkaline phosphatase(AP) after balloon injury. Lipid deposit,gene expression and infiltration of ApoB and macrophage were analyzed by oil red O(ORO) staining and immunochemistry,respectively.Results:Sub-endothelial lipids in the injured carotid artery (ORO positive) were significantly in the group of rabbits receiving LPL. At or near the sites where LPL was over-expressed,ApoB and macrophages were also positively stained. There was no significant change in plasma cholesterol,triglycerides and high-density lipoprotein cholesterol between the 2 groups.Conclusion: Overexpression of LPL in balloon injured carotid artery of rabbits can promote early atherosclerotic lesion formation.