被动吸烟对神经管上皮中凋亡相关基因bcl-2和Bax表达的影响

目的研究被动吸烟致畸中细胞凋亡的发生及其与凋亡相关基因Bcl-2和Bax表达的关系.方法采用自制的半封闭式被动吸烟染毒箱,用烟熏的方法建立金黄地鼠被动吸烟的致畸模型.用免疫组织化学和图象分析方法对鼠胚神经上皮Bcl-2和Bax表达进行了定性、定位和定量检测.结果1.Bcl-2和Bax正常分布于金黄地鼠胚胎神经上皮和周围间充质中,定位于核膜和胞浆,二者的表达量在孕9天最高,之后逐渐下降.2.被动吸烟后各实验组胚胎神经管上皮Bcl-2免疫组化染色强度均不同程度的低于对照组.Bax的表达均不同程度的高于对照组.结论被动吸烟能抑制Bcl-2的表达,促进Bax的表达,从而导致了过量细胞凋亡的产生.

Effect of passive smoking on expression of Bcl - 2 and Bax in embryonic neural epithelium

Objective: To study the passive smoking-induced teratogenesis on the expression of Bcl-2 and Bax. Methods: By using self-made passive smoking cage, the gold hamsters passive smoking model were established. Then the expression of Bcl-2 and Bax were investigated by immunohistochemistry and image analysis. Results: The Bcl-2 and Bax gene were expressed in the membrane of nuclei and cytoplasm in the neuroepithelial and mesenchymal cells, which reached the highest on the 9 day of pregnancy,and then decreased gradually. The expression of Bcl-2 was weaker in experimental group than control group, but the expression of Bax was more intense in experimental group than control group. Conclusion: Passive smoking can decrease the expression of Bcl-2 and increase the expression of Bax.