| 重组人乳头瘤病毒16型E7抗原痘苗病毒的构建 |
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| 引用本文： | 邱小萍,伍欣星,谭云,郑义,赵文日,赵文先,戴天力. 重组人乳头瘤病毒16型E7抗原痘苗病毒的构建[J]. 武汉大学学报(医学版), 2001, 22(2): 101-103 |
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| 作者姓名： | 邱小萍 伍欣星 谭云 郑义 赵文日 赵文先 戴天力 |
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| 作者单位： | 武汉大学基础医学院病毒学研究所, |
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| 摘 要： | 目的 :研究人乳头瘤病毒 16型E7(HPV16E7)基因蛋白的生物学和免疫学活性。方法 :用聚合酶链反应(PCR)技术扩增并分离出HPV16E735 9bp的基因片段 ,经XhoⅠ和BglⅡ双酶切后 ,定向插入到表达质粒PJ12 0的晚期启动子P11下游。结果 :经PCR技术、双酶切分析证明HPV16E7基因已克隆到载体PJ12 0上。结论 :HPV16E7基因痘苗病毒重组体构建成功 ,为其在真核细胞的表达奠定了基础
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| 关 键 词： | 乳头状瘤病毒,人致病力 病毒蛋白类分析 聚合酶链反应 克隆,分子 基因,病毒 |
| 修稿时间： | 2000-04-18 |
Construction of Recombinant HPV16 E7 Antigen Vaccinia Virus |
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| Qiu Xiao ping,Wu Xin xing,Tan Yun,et al Virus Research Institute,Basic Medical College,Wuhan University,Wuhan ,China. Construction of Recombinant HPV16 E7 Antigen Vaccinia Virus[J]. Medical Journal of Wuhan University, 2001, 22(2): 101-103 |
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| Authors: | Qiu Xiao ping Wu Xin xing Tan Yun et al Virus Research Institute Basic Medical College Wuhan University Wuhan China |
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| Affiliation: | Qiu Xiao ping,Wu Xin xing,Tan Yun,et al Virus Research Institute,Basic Medical College,Wuhan University,Wuhan 430071,China |
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| Abstract: | To study the biological and immunological properties of HPV16 E7 protein. Methods:HPV16 E7 gene fragment was amplified form plasmid of HPV16 E7 using PCR and digested by two restriction endonucleaes Xho I and Bgl Ⅱ. Results: E7 gene was orientally inserted into the downstream of late promotor P11 of expression vector PJ120.Conclusion:E7 gene has been successfuly cloned the site of the expressive vector PJ120.It is the beginning for the recombinant to express protien product in eukaryotic cell. |
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| Keywords: | papillomavirus human/PY virus proteins/AN polymerase chain reaction cloning molecular genes virus |
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