川芎嗪对肠粘膜屏障功能的保护作用

目的:探讨川芎嗪对失血性休克再灌注后肠粘膜屏障功能的保护作用。方法:30只日本大耳白兔随机分为3组:假手术组(A组),单纯休克组(B组),川芎嗪治疗组(C组)。以硫代巴比妥酸比色法、放射免疫法及硝酸还原酶法测定末端回肠粘膜丙二醛(MDA)、肿瘤坏死因子(TNFα)、白细胞介素-1β(IL-1β)及一氧化氮代谢产物(NO^-2/NO^-3)含量,并取体循环血做细菌培养,取肠粘膜行光镜和电镜观察。结果:B组肠粘膜MDA、TNFα、IL-1β含量明显高于A组,NO^-2/NO^-3浓度显著低于A组,而C组变化无显著;B组细菌移位率明显高于A组,而C组与A组比较无显著差异;B组肠粘膜损伤明显重于A组,C组明显轻于B组,而C组与A组差异无显著。结论:川芎嗪能保护失血性休克再灌注后肠粘膜屏障功能,这可能与其清除体内氧自由基、提高一氧化氮水平及阻抑炎性介质作用有关。

Protective effect of Ligustrazini on gut barrier function

AIM:To study the protective effect of Ligustrazini(LGT) on gut barrier function after hemorrhagic shock-reperfusion. METHODS: Thirty white rabbits were divided randomly into 3 groups: control group (A),shock group (B) and LGT group (C). Malondialdehyde(MDA), tumor necrosis factor-α(TNF_α), interleukin-1β(IL-1β) and nitric oxide products(NO₂^-/NO₃^-) contents were measured in intestinal mucosa at 3 hours following reperfusion,culture of bacteria in blood from rabbits of 3 groups was carried out,the intestinal mucosa was examined under optical and electron microscope. RESULTS: MDA, TNF_α, IL-1β and NO₂^-/NO₃^- contents of intestinal mucosa remained unchanged in group C,but increased significantly in group B, compared with group A. Incidence of bacterial translocation in group B was markedly higher than that in group A at 30 min following reperfusion,there was not any difference between group A and group C. Under light and electronic microscope,in comparison with A and C groups,intestinal mucosa damage in B group became more severe. CONCLUSION: LGT can protect gut barrier from intestinal ischemia-reperfusion injury induced by hemorrhagic shock through reducing oxygen free radicals,raising nitric oxide and preventing inflammation.