| 人参皂苷对K562细胞增殖与分化相关基因表达的影响 |
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| 引用本文： | 危建安,胡永珍,韩凌.人参皂苷对K562细胞增殖与分化相关基因表达的影响[J].广东药学院学报,2011,27(3):296-298. |
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| 作者姓名： | 危建安 胡永珍 韩凌 |
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| 作者单位： | 1. 广州中医药大学,第二临床医学院,中心实验室,广东广州510006
2. 广州中医药大学,第二临床医学院,血液科,广东广州510006 |
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| 基金项目： | 广州中医药大学创新基金 |
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| 摘 要： | 目的 探讨人参皂苷影响K562细胞增殖、分化的可能分子机制.方法 采用real-time PCR技术和SYBR Green染料法检测人参皂苷(20 μg/mL)作用于K562细胞36 h时间点Lif、Ccnd1、Mdm2、Erbb2、p53和myc等6个基因mRNA相对表达水平.结果 人参皂苷能显著促进K562细胞Li...
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| 关 键 词： | 人参皂苷 K562细胞 增殖 分化 基因表达 |
The effect of ginsenoside on mRNA expression of proliferation and differentiation-related genes in K562 cells |
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| WEI Jian-an,HU Yong-zhen,HAN Ling.The effect of ginsenoside on mRNA expression of proliferation and differentiation-related genes in K562 cells[J].Academic Journal of Guangdong College of Pharmacy,2011,27(3):296-298. |
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| Authors: | WEI Jian-an HU Yong-zhen HAN Ling |
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| Institution: | 1(1.The Central Laboratory;2.Hematology Department,The Second Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China) |
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| Abstract: | Objective To explore the potential mechanisms of ginsenosides on K562 cell proliferation and differentiation.Methods Relative quantification RT-PCR with SYBR Green dye was used to determine the expression of Lif,Ccnd1,Mdm2,Erbb2,p53 and myc mRNA in K562 cells treated with ginsenoside（20 μg/mL） at 36 h.Results mRNA expression of Lif was significantly up-regulated（P〈0.05）,and that of myc was markedly down-regulated（P〈0.05） in K562 cells when treated with traginsenoside.mRNA levels of Mdm2,Erbb2 and Ccnd1 were not significantly changed after treatment of ginsenoside（P〉0.05）.mRNA expression of p53 was very weak in K562 cells,so that was not statistically analysed.Conclusion The effect of ginsenoside on proliferative inhibition and differential induction of K562 cells is related to up-regulation of Lif mRNA and down-regulation of myc mRNA respectively. |
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| Keywords: | ginsenoside K562 cell line proliferation differentiation gene expression |
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