In vitro generation of tumor specific T cells that recognize a shared antigen of AML: molecular characterization of TCR genes |
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Authors: | Coppage Myra Belanger Todd Zauderer Maurice Sahasrabudhe Deepak |
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Institution: | University of Rochester Department of Pathology and Laboratory Medicine, 601 Elmwood Avenue, Rochester, NY 14642, United States. Sara.Rollinson@manchester.ac.uk |
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Abstract: | The identification of immunologically relevant tumor antigens is hampered by the difficulty of generating tumor-specific cytotoxic T cells (CTL). We present data demonstrating in vitro induction of autologous acute myelogenous leukemia (AML)-specific CTL. The specific T cell receptor has been identified and cloned. The CTL demonstrated specific lysis to autologous tumor blasts, but not to autologous BLCL or the NK-sensitive target K562. The clone secreted GM-CSF, TNFa, and IFNg when stimulated with AML blasts from 3 of 11 patients or cell lines tested, but not with K562 or autologous B-LCL. These three AML samples share a single HLA Class I antigen, HLA-A24. The T cell receptor genes identified by molecular methods are Vbeta7.9-J2.3-Cbeta2 and Valpha17-J49-Calpha. |
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