Maternal plasma DNA sequencing reveals the genome-wide genetic and mutational profile of the fetus |
| |
Authors: | Lo Y M Dennis Chan K C Allen Sun Hao Chen Eric Z Jiang Peiyong Lun Fiona M F Zheng Yama W Leung Tak Y Lau Tze K Cantor Charles R Chiu Rossa W K |
| |
Affiliation: | Centre for Research into Circulating Fetal Nucleic Acids, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China. loym@cuhk.edu.hk |
| |
Abstract: | Cell-free fetal DNA is present in the plasma of pregnant women. It consists of short DNA fragments among primarily maternally derived DNA fragments. We sequenced a maternal plasma DNA sample at up to 65-fold genomic coverage. We showed that the entire fetal and maternal genomes were represented in maternal plasma at a constant relative proportion. Plasma DNA molecules showed a predictable fragmentation pattern reminiscent of nuclease-cleaved nucleosomes, with the fetal DNA showing a reduction in a 166-base pair (bp) peak relative to a 143-bp peak, when compared with maternal DNA. We constructed a genome-wide genetic map and determined the mutational status of the fetus from the maternal plasma DNA sequences and from information about the paternal genotype and maternal haplotype. Our study suggests the feasibility of using genome-wide scanning to diagnose fetal genetic disorders prenatally in a noninvasive way. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|