bFGF对兔眼视网膜的影响挫伤Muller细胞Vimentin表达

目的研究碱性成纤维细胞生长因子（bFGF）对兔视网膜挫伤后Mtiller细胞的影响。方法26只白兔通过3J能量自由落体的方式制作兔眼挫伤性视网膜病变模型，随机分为bFGF实验组、生理盐水对照组、单纯挫伤组、正常对照组。其中正常对照组2只（4只眼），单纯挫伤组4只（8只眼），bFGF实验组和生理盐水对照组各10只（20只眼）。bFGF实验组每2d玻璃体腔内注射bFGF2μg（10μL），生理盐水对照组注射生理盐水10μL。采用免疫组织化学染色检测视网膜挫伤后3h，1、3、7、14d各时间点Miiller细胞波形蛋白（Vimentin）的表达。结果bFGF实验组视网膜Muller细胞Vimentin的表达高于生理盐水对照组，且呈上升趋势，两组比较各时段差异均有统计学意义（P〈0．05）。结论bFGF可以增强视网膜Muller细胞Vimentin的阳性表达，加速Muller细胞的活化，促进损伤修复。

Effect of bFGF on the expression of Vimentin in retinal Miiller cells in experimental retina blunt trauma

Objective Reactive gliosis which can promote the recovery of retina function at early stage of trauma,and basic fibroblast growth factor（bFGF） can activate Muller cells to accelerate healing of blunt trauma. Present study was to investigate the effect bFGF on Vimentin expression in Mtiller cells in experimental rabbit retinal blunt trauma. Methods An animal model of retinal blunt trauma was established in 24 rabbits by contusion of 3 J with a free falling iron bar. The model rabbits were divided into bFGF group,normal saline control group,trauma group and 2 normal rabbits were as control group. 10 μL of bFGF （2 μg） was intravitreonsly injected in bFGF group and the equal volume of normal saline was used in the normal saline control group after 3 days of retinal blunt trauma at a 2-day interval. The experimental rabbits were sacrificed and eyeballs were enucleated in 3 hours,1 day,3 days,7 days and 14 days to evaluate the Vimcntin expression in Muller cells by immunochemistry. Results The expression of Vimentin in MUller cells was increased markedly whatever in the intensity or quantity and showed a time- depended manner in both bFGF group and normal saline group. The statistically significance differences were seen in Vimentin expression between bFGF group and normal saline group in 1 day,3 days,7 days and 14 days after retinal blunt trauma （P 〈 0.05,P 〈 0.01 ）. Conclusion bFGF can enhance the expression of Vimentin in Muller ceils and activate MUller cells to accelerate repairing of retinal blunt trauma.