Molecular genotyping of Indian blood group antigens amongst regular voluntary blood donors of Surat city,Gujarat, India |
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| Institution: | 1. Surat Raktadan Kendra & Research Centre, Surat, Gujarat, India;2. National Institute of Immunohematology (NIIH, Mumbai), Mumbai, Maharashtra, India;1. Department of Hematology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), Madrid, Spain;2. Department of Emergency, Instituto de Investigación Sanitaria San Carlos (IdISSC), Hospital Clínico San Carlos (HCSC), Madrid, Spain;3. Platelet Research Lab, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain;4. Dept. of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands;5. Dept. of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands;6. Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Münster, Germany;7. Department of Medicine, University of Oviedo, Oviedo, Spain;1. Department of Blood Transfusion, the Affiliated Hospital of Qingdao University, Qingdao, Shandong, China;2. Department of Pathogeny Biology, Basic Medicine College, Qingdao University, Qingdao, Shandong, China;3. Department of Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, Shandong, China;1. Istanbul Medipol University Medical School, Department of Hematology and BMT, Istanbul, Turkey;2. Memorial Bahcelievler Hospital Adult Hematology and BMT Clinic, Istanbul, Turkey;3. Baskent University, Dr. Turgut Noyan Teaching and Medical Research Center, Apheresis Unit, Adana, Turkey;4. Yeditepe University Faculty of Medicine, Department of Internal Medicine Division of Hematology, Istanbul, Turkey;5. Hacettepe University, Department of Hematology, Ankara, Turkey;6. Bursa Uludag University Medical School, Department of Internal Medicine, Division of Hematology, Bursa, Turkey;7. Dokuz Eylul University Medical School, Department of Internal Medicine, Division of Hematology, Izmir, Turkey |
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| Abstract: | BackgroundThere is paucity of data related to the prevalence of the rare blood group antigens amongst South Gujarat blood donor population due to unavailability and high cost of antisera. Therefore it is difficult to screen donors for such rare antigens by gold standard haemagglutination assay. The single nucleotide polymorphism (SNPs) of Ina and Inb antigens is the base of the PCR based detection methods that help to detect these alleles in regular voluntary blood donors.Materials & methodsBlood samples of 200 unrelated regular voluntary blood donors wee collected. DNA was extracted using phenol-chloroform method and genotyped for Indian (Ina/IN*01, Inb/IN*02) blood group alleles by Sequence Specific PCR. Ina antigen positivity was confirmed by serology test.ResultsFour donors were found heterozygous for Ina antigen i.e. In (a + b+) by SS-PCR and their Ina positivity were confirmed by in-house polyclonal Anti-Ina reagent. SS-PCR was standardized using known heterozygous sample of a blood donor. The frequency of Ina antigen (2.0 %) was higher than Caucasians, lower than Iranians and Arabs while comparable to those reported among Indians of Mumbai city.ConclusionIn absence or unavailability of antisera particularly for low frequency alleles like Ina, such PCR based method would be extremely helpful to prepare rare donor registry by screening blood donors’ at large scale. Red cells of Ina positive donors can be used as in-house reagent red cells for screening and identification of corresponding antibody. |
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| Keywords: | Rare blood group antigens Indian blood group system Molecular genotyping SS-PCR South Gujarat Blood donors |
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