PRM1、PRM2和TRF2在特发性弱精子症中的表达及其相关性研究

目的研究特发性少弱精子症患者精子中PRM1、PRM2和TRF2 mRNA和蛋白表达水平,并探讨其与精子运动的相关性。方法选取2017年1月至2017年12月期间于本院生殖中心治疗特发性少弱精子症患者43例作为研究对象(观察组),本院精子库的供精者为对照组。比较两组精液参数,并采用RT-PCR法检测PRM1、PRM2和TRF2的mRNA水平;利用Western blot检测其蛋白水平。结果①观察组精子密度、精子活力及精子形态正常率均低于对照组(P<0.05);②观察组PRM1和PRM2 mRNA及蛋白相对表达量均显著低于对照组(P<0.05),而TRF2 mRNA及蛋白的相对表达量差异无统计学意义(P>0.05);③PRM1 mRNA与PRM2 mRNA与精子向前运动有显著的相关(P<0.05)。结论PRM1和PRM2与男性精子的运动能力相关,可能参与了特发性少弱精子症的发生;临床上可根据鱼精蛋白PRM1、PRM2的表达水平进行早期特发性少弱精子症的防治,为临床提供分子生物学机制,降低特发性少弱精子症的发生。

Expression and correlation of PRM1,PRM2 and TRF2 in idiopathic weak sperm disease

Objective To study the mRNA and protein expression levels of PRM1,PRM2 and TRF2 in sperm of patients with idiopathic oligospermia and to explore its correlation with sperm motility.Methods Forty-three patients with idiopathic oligozoospermia in the reproductive center of our hospital from January 2017 to December 2017 were selected as a research object.The sperm donors in our hospital were the control.The semen parameters of the two groups were compared,and the mRNA levels of PRM1,PRM2 and TRF2 were detected by RT-PCR.The protein level was detected by Western blot.Results①The sperm density,sperm motility and normal sperm morphology were lower in the idiopathic oligozoospermia group than in the normal group(P<0.05).②The relative expressions of PRM1 and PRM2 mRNA and protein in the idiopathic oligozoospermia group were significantly lower than those in the normal group(P<0.05),but the relative expression of TRF2 mRNA and protein was not statistically significant.③There was a significant correlation between PRM1 mRNA and PRM2 mRNA and sperm forward movement(P<0.05).Conclusions PRM1 and PRM2 are related to the motility of male sperm and may be involved in the development of idiopathic oligospermia.The prevention and treatment of early idiopathic oligozoospermia can be carried out according to the expres-sion level of protamine PRM1 and PRM2,which provides molecular biological mechanism and reduces the occurrence of idiopathic oligospermia.