| 原位PCR和免疫组化检测垂体腺癌P16基因 |
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| 引用本文： | 易静,陈玉英,于德蓉,李骁雄,熊文浩,沈建康.原位PCR和免疫组化检测垂体腺癌P16基因[J].上海交通大学学报(医学版),1999(3). |
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| 作者姓名： | 易静 陈玉英 于德蓉 李骁雄 熊文浩 沈建康 |
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| 作者单位： | 上海第二医科大学细胞生物学实验室!2O0025(易静，陈玉英，于德蓉)，上海第二医科大学附属仁济医院神经外科!2OO001(李骁雄，熊文浩，沈建康) |
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| 基金项目： | 卫生部科研基金!98－1－303 |
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| 摘 要： | 目的研究人垂体腺瘤P16基因的改变，同时探索原位PCR技术的适宜条件和结果确认，以及用于基因缺失检测的可行性。方法原位PCR和免疫组化技术。结界绝大部分间质细胞为P16蛋白免疫组化阴性，而一部分垂体肿瘤细胞呈P16阳性；针对P16基因的原位PCR信号则可见于垂体肿瘤细胞和间质细胞等各种细胞，即P16组化阴性的细胞同样表明有P16基因存在。结论原位PCR可以是P16基因研究的一种有效手段，提示垂体腺癌的P16基因改变形式可能主要是表达过度而不是基因缺失。
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| 关 键 词： | 原位PCR 免疫组化 P16基因 垂体腺瘤 |
In Situ PCR and Immunohistochemical Detection of P16 Gene in Pituitary Adenomas |
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| Yi Jing, Chen Yuying,et al.In Situ PCR and Immunohistochemical Detection of P16 Gene in Pituitary Adenomas[J].Journal of Shanghai Jiaotong University:Medical Science,1999(3). |
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| Authors: | Yi Jing Chen Yuying |
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| Abstract: | Objectivc To study Pl6 gene alterati0n in 31 cases of human pituitaryadenoma and to optimize the conditions for in situ PCR. Methods In situ PCR andimmun0histochemistry were used. Rcsults Part of pituitary tumor cells were positive for P16 immunostaining whereas the majority of stromal cells were P16 - negative. Signal of geneamplification in situ was displayed in both tumor cells and stromal cells regardless they wereimmunohistochemically positive or negative, indicating the presence of P16 gene in thesecells. Conclusion In situ PCR might be a helpful approach in study of P16 gene. Theresults suggested that the alteration of P16 gene in pituitary adenoma could beoverexpression rather than deletion. |
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| Keywords: | In situ PCR immunohistochemistry P16 gene pituitary adenomas |
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