| HPLC法测定碧玉珠中人参皂苷Rg1的含量 |
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| 引用本文： | 李华,刘潇潇. HPLC法测定碧玉珠中人参皂苷Rg1的含量[J]. 中国民族民间医药杂志, 2014, 0(4): 12-13 |
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| 作者姓名： | 李华 刘潇潇 |
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| 作者单位： | 广东省药品检验所,广东广州510180 |
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| 摘 要： | 目的:建立高效液相色谱(HPLC)法测定碧玉珠中人参皂苷Rg1的含量。方法:HPLC法采用Merck C18(5μm,150×4.6mm)柱,以乙腈-水(19:81)为流动相,流速为1 ml/min,柱温为20℃,检测波长为203 nm。结果:人参皂苷Rg1在0.1842~3.6840μg范围内与其峰面积呈良好的线性关系,r=0.9998,平均回收率为101.30%。结论:本方法简便、准确、可靠,可用于碧玉珠的质量控制。
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| 关 键 词： | HPLC 碧玉珠 人参皂苷Rg1 |
Determination of Ginsenoside Rgl in Biyuzhu Capsule by HPLC |
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| Affiliation: | Li Hua ,Liu Xiao- xiao (Guangdong Institute for Drug Control, Guangdong Province, Guangzhou 510180, China) |
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| Abstract: | Objective To establish a HPLC method for determine ginsenoside Rgl in Biyuzhu Capsule. Methods The HPLC was applied with the condition as follows： column： MerckMerck C18（5μm,150×4.6mm）, mobile phase： ACN -H20 （19：81 ） . flow speed： 1.0 ml · min^-1, the column temperature was 20℃, detection： 203 nm. Results The linear range of ginsenoside Rgl was 0. 1842 -3. 6840μg, r =0. 9998. The average recovery of ginsenoside Rgl was 101.9%. Conclusion The method is simple, accurate and specific, it can be used for quality control of Bivuzhu CaPsule. |
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| Keywords: | HPLC Biyuzhu Capsule Ginsenoside Rg1 |
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