小脑顶核电刺激对小鼠脑撞击伤后皮质神经再生的促进作用

目的探讨小脑顶核电刺激(fastigial nucleus stimulation,FNS)对小鼠脑撞击伤(brain impact injury,BII)后皮质神经再生的促进作用。方法将54只昆明小鼠分为假手术组(n=7)、BII组(n=21)、FNS组(n=7)及FNS+BII组(n=19)。假手术组仅行皮肤切开和颅骨分离,但不撞击脑组织。BII组复制小鼠自由落体脑撞击伤,FNS组单纯行FNS。FNS+BII组在FNS后24 h复制小鼠自由落体脑撞击伤。比较BII组和FNS+BII组伤后的临床运动障碍评分。采用实时荧光定量PCR技术,观察各组小鼠脑撞击伤病灶边缘区生长相关蛋白43(GAP-43)mRNA表达的改变。结果 FNS+BII组临床运动障碍评分在颅脑损伤后3、7、14 d较BII组无明显降低(均P>0.05)。实时荧光定量PCR结果显示:FNS+BII组GAP-43 mRNA表达水平在伤后7 d为(2.69±0.86)pg/μg RNA;BII组为(1.55±0.72)pg/μg RNA,明显低于FNS+BII组(P<0.05)。结论 FNS能显著增加撞击伤后GAP-43 mRNA的表达,促进神经纤维再生,但未见神经运动功能的明显改善。

Promoting effect of cerebellar fastigiai nucleus stimulation on the cortex neural regeneration after impact brain injury in mice

Objeeive To explore the promotion effect of cerebellar fastigial nucleus stimulation （FNS） on the cortex neural regeneration after brain impact injury （BII） in mice. Methods Fifty-four mice were divided into sham-operated group （n = 7）, BII group （n = 21）, FNS group （n = 7） and FNS＋BII group （n = 19）. Skin incision and skull detaching were performed in sham-operated group without BI/Weigh-drop brain impact injury was replicated in BII group. FNS was performed in FNS group. FNS was performed and 24 h later brain impact injury was induced using weigh-drop method in FNS＋BII group. The clinical movement disorder score was evaluated in BII group and FNS＋BII group after BII. The expression of growth associated protein 43 （GAP-43） mRNA in the marginal zone of BII lesion was detected by real-time PCR in all the groups. Results There was no significant decrease of clinical movement disorder score in FNS＋BII group than that in BII group 3, 7, 14 days after BII （all P 〉 0.05）. GAP-43 mRNA expression was 2.69±0.86 pg/μg RNA 7 days after BII in FNS＋BII group, which was significantly higher than 1.55±0.72 pg/μg RNA in BII group （P 〈 0.05）. Conclusions FNS can significantly increase the expression of GAP-43 mRNA and promote the regeneration of neural fiber in BII mice without the improvement of neurologic movement function.