IL-21在慢性乙型肝炎中免疫调节作用的研究

目的探讨白细胞介素21(IL-21)在慢性乙型肝炎中的免疫调节作用。方法采用酶联免疫吸附试验(ELISA)检测32例慢性乙型肝炎病人(病人组)和29例健康人(对照组)血清中IL-21水平。提取外周血单个核细胞后分别设为IL-21刺激组、IL-2刺激组和未刺激组,多色流式分析技术检测CD8+T细胞频率,胞内细胞因子染色技术检测CD8+IFN-γ+T细胞的频率。结果与对照组相比,病人组血清中IL-21浓度明显增高(t=2.75,P<0.01);与IL-2刺激组和未刺激组相比,IL-21刺激组CD8+T细胞频率及分泌IFN-γ水平显著升高(F=59.27、10.27,q=3.82~14.11,P<0.01)。结论 IL-21通过对CD8+T细胞的免疫调节效应参与慢性乙型肝炎病人体内病毒的清除,为临床治疗提供了新的靶标。

STUDY ON IMMUNE REGULATION OF IL-21 IN CHRONIC TYPE B HEPATITIS

Objective To investigate the role of IL-21 in patients with chronic hepatitis B(CHB). Methods Serum IL-21 in 32 patients with CHB and 29 healthy subjects were determined using enzyme-linked immunosorbent assay(ELISA).After extraction of peripheral blood mononuclear cell,a setting of IL-21-stimulus,IL-2-stimulus and not-yet stimulus group was made.HBV DNA was analyzed with RT-PCR.The frequency of CD8+ T cells was analyzed by flow cytometry,and that of IFN-γ by intracellular cytokine straining. Results Compared with the controls,the serum concentration of IL-21 in CHB was markedly higher(t=2.75,P<0.01);compared with IL-2 stimulus and not-yet stimulus,the frequency of CD8+ T cells and secretion of CD8+IFN-γ+ T cells dramatically increased(F=59.27,10.27;q=3.82-14.11;P<0.01). Conclusion IL-21 involves in elimination of viruses in the body of patients with CHB through immunological regulation of CD8+ T cells,which provides a new target for clinical therapy of the disease.