| 反相高效液相色谱法测定活骨胶囊中丹参酮ⅡA含量 |
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| 引用本文： | 孙彩华,钱松洋,陈水英. 反相高效液相色谱法测定活骨胶囊中丹参酮ⅡA含量[J]. 中国药业, 2007, 16(7): 16-17 |
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| 作者姓名： | 孙彩华 钱松洋 陈水英 |
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| 作者单位： | 1. 浙江中医药大学附属第一医院药剂科,浙江,杭州,310004
2. 正大青春宝药业有限公司,浙江,杭州,310023 |
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| 摘 要： | 目的建立测定活骨胶囊中丹参酮ⅡA含量的反相高效液相色谱法（RP-HPLC法）。方法采用Symmetry C18色谱柱（250mm×4.6mm,5μm）,以甲醇-水（80∶20）为流动相,流速为1.0mL/min,检测波长为270nm,柱温为室温。结果丹参酮ⅡA进样量在0.08～0.80μg范围内与峰面积线性关系良好,回归方程Y=4259836.7X-33874.1,r=0.9998,平均加样回收率为99.1%,RSD=1.5%（n=5）。结论RP-HPLC法简便、准确,重现性好,稳定可靠,可用于活骨胶囊的质量控制。
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| 关 键 词： | 高效液相色谱法 活骨胶囊 丹参酮ⅡA |
| 文章编号： | 1006-4931（2007）07-0016-02 |
| 修稿时间： | 2006-08-30 |
Determination of Tanshinone ⅡA in Huogu Capsules by HPLC |
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| Sun Caihua,Qian Songyang,Chen Shuiying. Determination of Tanshinone ⅡA in Huogu Capsules by HPLC[J]. China Pharmaceuticals, 2007, 16(7): 16-17 |
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| Authors: | Sun Caihua Qian Songyang Chen Shuiying |
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| Affiliation: | 1. Department of Pharmacy, the First Affiliated Hospital of Zhefiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang, China 310004; 2, Chiatai Qingchunbao Pharmaceutical Co., Ltd,, Hangzhou, Zhejiang, China 310023 |
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| Abstract: | Objective To establish a RP-HPLC method for determination of tanshinone ⅡA in Huogu capsules. Methods A Symmetry C18 （250 mm×4.6mm,5 μm） column was used. The methyl water （80：20） was as the mobile phase. TEe flow rate was 1.0 mL/min. TEe determination wavelength was at 270 nm and the column temperature was maintained at room temperature. Results TEe linear range was 0.08-0.80 μg for tanshinone ⅡA （r=0.9998） and the average recovery rate was 99.1%, RSD=1.5% （n=S）.The linear equation was Y=4 259 836. 7X-33 874. 1. Conclusion The method is simple, accurate, reproducible, reliable. It can be used for the quantitative control of Huogu capsules. |
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| Keywords: | HPLC Huogu capsules tanshinone ⅡA |
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