快速分析测定大豆甾醇侧链降解底物的方法

建立了快速分析测定大豆甾醇侧链降解过程中底物大豆甾醇和产物雄甾-4-烯-3，17-二酮(AD)及雄甾-1，4-二烯-3，17-二酮(ADD)的方法。采用薄板层析(TLC)，在板层为硅胶60GF254，流动相为石油醚-乙酸乙酯(6：4)的条件下，成功的分离了底物与产物，从而能快速简便的监测底物的消耗与产物的生成情况。另外根据底物与产物AD与ADD在紫外区的吸收情况，利用254nm与298nm双波长测定ADD与AD的生成量。上述两种方法便于工厂对生产情况的同步检测。

The Methods for Rapid Analysis and Determination the Cleavage Products of Soybean Sterol

The methods for rapid analysis and determination the cleavage of soybean sterol, in which the soybean sterol was degraded and the products Androst 4 ene 3,17 dione(AD) and Androst 1,4 diene 3,17 dione(ADD) were produced, were described in this paper. With thin layer chromatography, the soybean sterol AD and ADD were succeed to divide. So the consumption of substrate and the produce of products can be monitored rapidly and conveniently. And according to the absorption of substrate and products in ultraviolet, the amount of ADD and AD can be assayed in two wavelength. The aforesaid two methods were convenient to manufactory to assay produce simultaneously.