骨髓基质细胞与可注射聚丙交酯多孔微载体共培养的体外实验研究

目的体外共培养骨髓基质细胞与聚丙交酯多孔微载体,研究微载体对骨髓基质细胞的增殖、凋亡等生理特性的影响,为细胞移植治疗提供一种安全可靠的载体。方法密度梯度离心法获取大鼠骨髓基质细胞,与可注射聚丙交酯多孔微载体在体外共培养,倒置显微镜及扫描电镜下观察细胞的生长及黏附在微载体的情况,并应用MTT法、流式细胞仪比较共培养、单独培养以及黏附在微载体的细胞的增殖及凋亡等生理特性。结果两者共培养时,骨髓基质细胞可长期存活、增殖,并可以黏附于微载体的表面及内部孔洞中,共培养时微载体对骨髓基质细胞的增殖、凋亡等特性无明显影响。结论可注射聚丙交酯多孔微载体是一种安全、可靠的细胞载体。

Experimental Study on Co-culture of Bone Marrow Stromal Cells and Fabrication of Injectable Polylactide Porous Microcarriers in Vitro

Objective To research the bone marrow stromal cells＇biological abilities to proliferate and apoptosis though co-culturing it and a kind of injectable poly-D、L-lactic acid porous scaffold microcarriers in vitro,We shall providing a kind of safety carrier that can carry cells in treating the disease of central nervous system applying cells transplantation strategy.Methods Harvest bone marrow stromal cells,applying density and gradient centrifugation,and research it′s activity、proliferation and adhering to microcarriers under inverted microscope and scanning electron microscope though co-culturing it and a kind of injectable poly-D、L-lactic acid porous scaffold microcarriers in vitro.Detecting the cells′proliferation and apoptosis among the cells of co-culturing,single culturing and adhering to microcarriers by means of MTT and flow cytometry.Results The bone marrow stromal cells can long-term surviving and proliferating and adhering to surface and internal cavitas of microcarriers in co-culturing in vitro.The microcarriers is not obviously influence to the cells′biological abilities when co-culturing.Conclusion The injectable poly-D、L-lactic acid porous scaffold microcarriers is a kind of safety carrier in cells transplantation strategy.