生存素与半胱氨酸天冬氨酸酶在子宫内膜异位症的表达及发病机制研究

目的研究生存素（Survivin）和半胱氨酸天冬氨酸酶（Caspase-3）蛋白在子宫内膜异位症（endo-metriosis,简称内异症）发生发展中的作用。方法 2007年10月—2009年1月在郑州市第一人民医院,以内异症患者的异位内膜及在位内膜各36例以及正常子宫内膜30例为研究对象,采用免疫组化法检测Survivin及Caspase-3蛋白在各组中的表达,并分析Survivin和Caspase-3蛋白在各组中免疫组化染色的平均灰度值。结果①Survivin在内异症患者异位内膜及在位内膜的灰度值均高于正常对照组子宫内膜（P〈0.05）,其中异位内膜组的灰度值最高（P〈0.05）。②Caspase-3在内异症患者的在位内膜及异位内膜的灰度值均低于正常对照组子宫内膜（P〈0.05）,其中异位内膜组的灰度值最低（P〈0.05）。③内异症在位内膜及异位内膜中,Sur-vivin与Caspase-3的表达均呈负相关（P〈0.05）。结论子宫内膜异位症中Survivin的高表达可能抑制Caspase的活化,使子宫内膜细胞的凋亡与增殖平衡发生紊乱,两者共同参与了内异症的发生发展。

Relation between Survivin/Caspase-3 Gene and Pathogenesis of Endometriosis

Objective To research whether the endometriosis is associated with Survivin and Caspase-3 gene.Methods Immunohisto-chemistry method was used to detect the expression of Caspase-3 and Survivin gene in normal endometrial tissue（n=30）,ectopic and eutopic endometriotic tissue（n=36,respectively）.The study used the Biosens Digital Imaging System to exam the average gray value of production of Caspase-3 and Survivin positive reacitity.Results ①The expression of Survivin of eutopic group and ectopic group was higher than the normal control group（P〈0.05）.②The expression of Caspase-3 of eutopic group and ectopic group was weaker than the normal control group（P〈0.05）.③In eutopic and ectopic endometrium group,according to the relative analysis,the corelation of Survivin and Caspase-3 is reverse correlation（P〈0.05）.Conclusion The higher expression of survivin depress the activity of Caspase-3,which destroy the balance of breeding and apoptosis,and which play a key role in the pathogenesis of endometriosis.