Circulating B7-H3(CD276) Elevations in Cerebrospinal Fluid and Plasma of Children with Bacterial Meningitis |
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| Authors: | Xuqin Chen Guangbo Zhang Yan li Xing Feng Fengguo Wan Liya Zhang Jian Wang Xueguang Zhang |
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| Institution: | (1) Institute of Medical Biotechnology, Suzhou University, No.708, Renmin Road, Suzhou, 215007, China;(2) Department of Neurology, Children’s Hospital of Suzhou University, No.303, Jingde Rode, Suzhou, 215003, China;(3) Clinical Immunology Laboratory, Suzhou University No. 1 Affiliated Hospital, Suzhou, 96 Shizi Road, 215006, China;(4) Department of Newbone Medicine, Children’s Hospital of Suzhou University, No.303, Jingde Rode, Suzhou, 215003, China;(5) Institute of Pediatric Medicine, Suzhou University, No.303 Jingde Rode, Suzhou, 215003, China;(6) Clinical Laboratory, Children’s Hospital of Suzhou University, No.303, Jingde Rode, Suzhou, 215003, China; |
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| Abstract: | The objective of this study was to analyze the clinical significance of cerebrospinal fluid (CSF) and plasma concentrations
of B7-H3, tumor necrosis factor-alpha (TNF-α), gamma interferon (IFN-γ), and interleukin-17 (IL-17) in bacterial and aseptic
meningitis in children. The participants were six children with bacterial meningitis, 16 with aseptic meningitis, and 12 control
subjects. All participants were between 2 months and 12 years of age on admission. Cytokines determination was performed by
enzyme-linked immunosorbent assay technique. CSF and plasma-circulating B7-H3 were significantly higher in the bacterial meningitis
group as compared with the aseptic group (p = 0.001) and the control group (p = 0.000 and p = 0.001 respectively). However, CSF and plasma-circulating B7-H3 in aseptic meningitis were not significantly higher than
control group (p = 0.071 and p = 0.72 respectively).CSF and plasma-circulating TNF-α were significantly higher in the bacterial meningitis group as compared
with the aseptic group (p = 0.004 and p < 0.0001 respectively) and control group (p = 0.004 and p < 0.0001 respectively). Similarly, we did not observe significant elevated TNF-α levels in CSF and plasma in aseptic group
compared with control group (p = 0.03 and p = 0.12 respectively). IFN-γ levels in CSF and plasma were undetectable in control group, and we did not find statistical
significances in both of CSF and plasma between the elevated IFN-γ level in bacterial meningitis group and aseptic meningitis
group(p = 0.055 and p = 0.095 respectively) CSF and plasma levels of IL-17 were undetectable in all subjects. There were correlations between B7-H3
and TNF-α, IFN-γ (r = 0.875, p = 0.000; r = −0.693, p = 0.000, respectively) in CSF in meningitis subjects. In plasma, levels of B7-H3 in bacterial meningitis on admission correlated
positively with TNF-α (r = 0.968, p = 0.002), and white blood cell counts (r = 0.973, p = 0.001). Detectable CSF levels of B7-H3, TNF-α, and IFN-γ on admission were not associated significantly with any of CSF
characteristics. Additionally, CSF and plasma levels of B7-H3 decreased remarkably after treatment. Altogether, our data indicated
that circulating B7-H3 and TNF-α levels in the CSF and plasma were useful markers for distinguishing bacterial from aseptic
meningitis, and Circulating B7-H3 was demonstrated to be useful in evaluating the intensity of the infectious inflammatory
process in the central nervous system in children.
An erratum to this article can be found at |
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| Keywords: | B7-H3 Meningitis Cerebrospinal fluid (CSF) Plasma Children |
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