白芍总苷对葡萄膜炎模型大鼠的保护作用

目的:研究白芍总苷对葡萄膜炎模型大鼠的保护作用。方法:于大鼠足底部注射伤寒杆菌内毒素(LPS)200μg以复制大鼠葡萄膜炎模型。32只SD大鼠随机均分为正常对照(等容生理盐水)组、模型(对容生理盐水)组、白芍总苷(4.8 g/kg,每隔6 h给药1次,共3次)组、醋酸泼尼松(0.05 g/kg,给药1次)组,复制模型前1 h开始灌胃给药。用裂隙灯显微镜观察大鼠临床改变,通过病理学检查观察大鼠眼部组织学改变。结果:正常对照组未见炎症发生;与正常对照组比较,模型组炎症临床评分升高,差异有统计学意义(P<0.05),大鼠虹膜、睫状体、视网膜与玻璃体腔炎症细胞浸润程度明显加剧;与模型组比较,白芍总苷组炎症临床评分降低,差异有统计学意义(P<0.05),大鼠虹膜、睫状体、视网膜与玻璃体腔炎症细胞浸润程度明显改善。结论:白芍总苷对LPS诱导的葡萄膜炎大鼠有一定保护作用。

Protective Effects of Total Glucosides of Paeony on Uveitis Model Rats

OBJECTIVE： To study the protective effect of total glucosides of paeony （TGP） on uveitis model rats. METH- ODS ： Uveitis model was induced by LPS 200 μg via vola of SD rats. 32 SD rats were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, TGP group （4.8 g/kg, every 6 h, total of 3 times） and prednisone acetate group （50 mg/kg, once）. They were given relevant medicines 1 h before modeling. Slit lamp and his- tology were used to detect the change of ocular tissues at different time points after the induction. RESULTS： No uveitis was found in normal control group. Compared with normal control group, clinical score of model group increased significantly; there was sta- tistical significance（P〈0.05）. The infiltration of inflammatory cells into iris, ciliary body, retina, and vitreous body were aggravat- ed; compared with model group, clinical score of TGP group decreased significantly（P〈0.05）, while the infiltration of inflamma- tory cells into iris, ciliary body, retina, and vitreous body were improved significantly. CONCLUSIONS： TGP is effective for LPS-induced uveitis.