不同碘摄入量对子代大鼠脑神经递质及其相关酶活性的研究

目的通过研究子代大鼠脑组织中神经递质含量及相关酶活性,探讨不同碘摄入量对脑功能的影响。方法断乳后1月的W istar大鼠随机分为4组:低碘组,适碘组,10倍碘组,50倍碘组,分别饮用含不同碘浓度的水,饲养3月后随机交配,仔鼠饲养28 d后,断头取其大脑组织,称重,测定单胺氧化酶(MAO)和胆碱酯酶(ChE)活性。结果与适碘组相比,其它各组大鼠脑重量均明显降低(P<0.05或P<0.01),但低碘组最明显;20%脑组织匀浆中蛋白质含量,低碘组明显降低(P<0.01);低碘组仔鼠脑内MAO活性明显升高(P<0.01),ChE活性明显降低(P<0.01);50倍碘组大鼠MAO活性明显升高(P<0.01)。结论碘缺乏和严重碘过量可影响子代大鼠脑的神经递质及其相关酶活性,但碘缺乏的影响远比碘过量严重。

Study on the relationship between different iodine concentration and brain neurotransmitter and enzyme activity

Objective To study the relationship between different iodine concentration and brain development by measuring the neurotransmitter concentration and enzyme activity.Methods One-month weaned Wistar rats were divided into four groups,low iodine group;normal iodine group;ten-fold high iodine;fifty-fold high iodine;and fed with water of different iodine concentration by adding potassium iodide respectively.Rats mate randomly after three months.The baby rats were sacrificed after 28 days,then weigh brains and test the NO and the activity of NOS,MAO and ChE.Results Compared with the normal iodine group,the weight of brain in other groups are lower(P<0.05 or P<0.01),and the low iodine group is prominent;the content of protein in 20% brain decreased in low iodine group(P<0.01);in low iodine group,MAO increased(P<0.01),and ChE decreased(P<0.01);in fifty-fold high iodine group,MAO increased(P<0.01 or P<0.05).Conclusions Both iodine deficiency and iodine excess can affect the neurotransmitter and the related enzyme activity.However,the iodine deficiency is more serious than the iodine excess.