| 经癌胚抗原重组痘苗病毒转染的树突状细胞体外诱导特异性T细胞免疫 |
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| 引用本文： | 吴军,王晓怀,杨太成,冼江,郑文岭.经癌胚抗原重组痘苗病毒转染的树突状细胞体外诱导特异性T细胞免疫[J].南方医科大学学报,2002,22(3):256-258. |
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| 作者姓名： | 吴军 王晓怀 杨太成 冼江 郑文岭 |
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| 作者单位： | 1. 广州军区广州总医院放射肿瘤科
2. 广州军区广州总医院肿瘤分子生物学研究所 |
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| 摘 要： | 目的研究人癌胚抗原重组痘苗病毒(rV-CEA)转染外周血树突状细胞(DC)后能否在体外诱导CEA特异性细胞毒性T淋巴细胞免疫。方法将rV-CEA转染外周血单核细胞来源的DC后用于激发同源的T细胞,检测其对T细胞的增殖作用以及对CEA分泌性肿瘤细胞的杀伤活性,并与未经rV-CEA转染的DC激发的T细胞进行比较。结果经rV-CEA转染的DC激活的T细胞对CEA分泌性肿瘤细胞具特异性杀伤作用。结论rV-CEA转染的DC可以诱导CEA特异性T细胞活性。
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| 关 键 词： | 树突状细胞 癌胚抗原 痘苗病毒 T淋巴细胞 细胞增殖 |
| 文章编号： | 1000-2588(2002)03-0256-03 |
| 修稿时间： | 2001年10月15 |
Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro |
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| WUJun ,WANGXiao-huai ,YANGTai-cheng ,XIANJiang ,ZHENGWen-ling.Dendritic cells transfected with carcinoembryonic antigen-vaccinia recombinant virus induces CEA-specific immunity mediated by cytotoxic T lymphocytes in vitro[J].Journal of Southern Medical University,2002,22(3):256-258. |
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| Authors: | WUJun WANGXiao-huai YANGTai-cheng XIANJiang ZHENGWen-ling |
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| Institution: | WUJun 1,WANGXiao-huai 1,YANGTai-cheng 2,XIANJiang 2,ZHENGWen-ling 2 Departmentof Oncology 1,Instituteof MolecularOncology 2,GeneralHospitalof GuangzhouCommand,Guangzhou510010,China |
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| Abstract: | Objective To observewhetherdendriticcells(DCs)transfectedwithcarcinoembryonicantigen(CEA)-vaccinia recombinantvirus(rV-CEA)inducescytotoxicT lymphocyte-mediatedCEA-specificimmunityin vitro.Methods DCs derivedfromperipheralbloodmonocytesweretransfectedwithrV-CEAandthencoculturedwithautologousT cells.The proliferationof theinducedT cellsandtheircytotoxicactivityagainstCEA-secretingtumorcellswereassessedandcompared withthoseof T cellsinducedby DCswithoutrV-CEAtransfection.Results T cellsinducedby DCstransfectedwithrV-CEA showedspecificcytotoxicityagainstCEA-secretingtumorcells.Conclusion DCs transfectedwithrV-CEAcan elicit activationof CEA-specificT cells. |
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| Keywords: | dendriticcells carcinoembryonicantigen vacciniavirus T lymphocytes cellproliferation |
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