MMP-9在人非小细胞肺癌中的表达及其与转移和预后的关系

目的检测基质金属蛋白酶-9(MMP-9)在非小细胞肺癌(NSCLC)中的表达,并探讨其表达水平与转移和预后的关系。方法采用免疫组化(Elivision法)检测85例非小细胞肺癌原发灶、35例癌旁组织、39例肺良性病变和56例淋巴结转移灶中MMP-9的表达水平,用图像分析方法测定其积分光密度(IOD),以IOD值表示MMP-9的表达水平,并分析其与术后5年生存率的关系。结果(1)非小细胞肺癌MMP-9的IOD值(10.7±1.9)显著高于癌旁组织组(7.1±2.3)和良性病变组(7.5±2.2)(P<0.05);(2)淋巴结转移灶组(15.8±2.6)表达显著高于原发灶组织(10.7±1.9)(P<0.05);(3)MMP-9在原发灶组织中的表达水平与肿瘤大小、TNM分期、组织学类型、淋巴结转移密切相关(P<0.05),而与性别、年龄、肿瘤分化程度无关(P>0.05);(4)MMP-9高表达组患者的5年生存率(4.7%)显著低于MMP-9低表达组(19.0%)(P<0.05)。结论MMP-9的表达与肿瘤侵袭和转移密切相关,检测NSCLC中MMP-9的表达水平在评估患者预后方面具有一定的价值。

The relationship between the expression of MMP-9,the metastasis and prognosis in non small cell lung cancer

Objective To investigate the expression of metalloproteinases-9 （MMP-9） in non small cell lung cancer（NSCLC） and analyze the relationship with metastasis and prognosis. Methods The expressions of MMP-9 were detected with immunohistochemistry （Elivision） in 85 tissues from primary lesion of NSCLC cases, 35 para tumor tissues, 39 cases of benign lesion, and 56 cases of lymph nodes metastasized. Integral optical density （IOD）were determined by image analysis system, and the expression of MMP-9 was presented with IOD value, then analyse its correlation with 5-year survival rate. Results The IOD was significantly higher in NSCLC than in para tumor tissue and benign lesion tissue （ 10.7 ± 1.9 vs 7.1 ± 2.3 and 7.5 ± 2.2, P 〈 0.05）. The expression of MMP-9 displayed higher in metastasis lymph node than primary lesion（ 15.8 ± 2.6 vs. 10.7 ± 1.9, P 〈 0.05 ）. The expression of MMP-9 had positive correlation with tumor size,TNM stage, histological type, metastasis（ P 〈 0.05）. However, it had no correla- tion with gender, age, and tumor differentiation （ P 〉 0.05 ）. The 5-year survival rates with strong expression of MMP-9 were significantly lower than those patients with weak expression （4.7 % vs 19.0%, P 〈 0.05 ）. Conclusion It demonstrated that the expression of MMP-9 in NSCLC might be related to invasion and metastasis and might be contribute to the prognostic judgment.