紫七软肝片对大鼠肝纤维化湿热瘀毒证模型转化生长因子-β1和基质金属蛋白酶组织抑制剂-1表达的动态影响

目的：探讨紫七软肝片对复合因素致大鼠肝纤维化湿热瘀毒证模型作用的动态影响。方法：采用复合因素（CCl4＋高脂饮食＋酒精）干预大鼠,分正常组、模型组、紫七软肝片治疗组和秋水仙碱对照组,分别观察第4周和8周大鼠肝纤维化相关指征的变化,检测其血清透明质酸（HA）、层粘连蛋白（LN）、Ⅲ型前胶原氨端肽（PⅢP）以及采用肝组织免疫组化检测其转化生长因子-β1（TGF-β1）和基质金属蛋白酶组织抑制剂-1（TIMP-1）的动态表达情况。结果：模型组大鼠从4周和8周末HA、LN、PⅢP以及TGF-β1和TIMP-1均有不同程度的改变,并呈现动态加重的过程,而紫七软肝片和秋水仙碱均有明显的治疗作用,且紫七软肝片的作用更为明显（P〈0.05或P〈0.0l）。结论：抑制纤维组织增生、拮抗TGF-β1和TIMP-1表达等是紫七软肝片有效防治肝纤维化的重要机制。

Ziqi Ruangan tablet' s influence on TGF-β1 and TIMP-1 expressed in rat model for hepatic fibrosis with damp heat and toxic syndrome

Objective： To estimate Ziqi Ruangan tablet＇s influence on TGF-β1 and TIMP-1 expressed in the rat model for hepatic fibrosis with damp heat and toxic syndrome. Methods： Rats, fed with CCl4, high fat and alcohol, were divided into four groups, normal group and model group, Ziqi Ruangan tablet group and colchieine one. Investigate rats＇ signs changes and detect the levels of HA, LN, PⅢP, TGF-β1, TIMP-1 after 4 weeks and 8 weeks, respectively. Results： The levels of HA, LN, PIIIP, TGF-β1, TIMP-1 were varied in different degree, and become devastated even more with the disease progressed. Rats in groups of Ziqi Ruangan tablet and colchieine were in better condition, especially in group of Ziqi Ruangan tablet （P 〈 0.05 or P 〈 0. 01 ） . Conclusion： The mechinism of Ziqi Ruangan tablet＇s effect on the hepatic fibrosis lies in prohibiting fibrosis hyperplasia, restricting expressing of TGF-β1 and TIMP-1.