HPLC法测定丙戊酸的血药浓度

目的：建立快速柱前衍生-高效液相色谱法测定丙戊酸（VPA）血药浓度的方法。方法：选择ω-溴苯乙酮为衍生试剂，环己烷羧酸为内标，Hypersil ODS-C18柱（250mm×4．6mm，5μm）为分析柱；流动相为甲醇-水（75：25，V／V），检测波长为248nm，流速为1．0ml／min。血样经酸化后以二氯甲烷提取。结果：内标环己烷羧酸和VPA的保留时间分别为8．0min和12．8min，线性范围为25～200μg／ml，平均回收率为100．61％，日内、日间RSD均小于4．19％。结论：本法快速、简便、准确，适合临床常规监测需要。

HPLC determination of plasma concentrations of valproic acid

Objective： To establish a rapid pre-column derivatization-high-performance liquid chromatography determination of valproic acid （VPA） plasma method. Methods： ω-bromoacetophenone as a derivative reagent, eyclohexane carboxylic acid as the internal standard, Hypersil ODS-C18 column （250mm×4.6mm,5μm） for the analytical column; mobile phase was methanol-water （75：25, V/V）, detection wave length was 248 nm, flow rate was 1.0 ml/min. Blood extracted by dichloromethane after acidification. Results： The internal standard cyclohexane carboxylic acid and VPA respectively, the retention time of 8.0 min and 12.8 min, the linear range of 25-200 μg/ml, the average recovery was 100.61%, days, day RSD were less than 4.19%. Conclusion： The method is rapid, simple, accurate and suitable for routine monitoring of clinical need.